Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex

Hsiang Ling Ho; Yu Shih Shiau; Mei Yu Chen

doi:10.1007/s00294-005-0570-8

Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex

Hsiang Ling Ho, Yu Shih Shiau, Mei Yu Chen^*

^*Corresponding author for this work

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Saccharomyces cerevisiae TSC11/AVO3 is an essential gene encoding one component of TORC2, a multi-protein complex of yeast Tor2p that also contains Lst8p, Avo1p, and Avo2p. Despite the proven physical association among TORC2 components, little is known about the functional linkage or cellular pathways these proteins act in. Here, we present genetic data linking the function of TSC11 to the regulation of cell integrity. Mutants carrying temperature-sensitive (ts) alleles in different regions of TSC11 displayed cell wall defects, evidenced by characteristic osmotic stabilizer-remediable cell lysis, susceptibility to trypan blue staining, and sensitivity to cell wall-digesting enzymes. Dosage suppression analysis identified different groups of genes in rescuing phenotypes of different tsc11^ts mutants. AVO1 suppressed one class of mutants, whereas active PKC1, AVO2, and SLM1 partially rescued another. Our findings demonstrate functional connections among TORC2 components and we speculate that Tsc11p exerts its function via a Pkc1p-independent mechanism mediated through Avo1p, and a Pkc1p-dependent mechanism mediated through Avo2p and Slm1p.

Original language	English
Pages (from-to)	273-288
Number of pages	16
Journal	Current Genetics
Volume	47
Issue number	5
DOIs	https://doi.org/10.1007/s00294-005-0570-8
State	Published - May 2005

Keywords

Actin
Cell wall
Multicopy suppressor
Protein kinase C
Target of rapamycin

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10.1007/s00294-005-0570-8

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title = "Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex",

abstract = "Saccharomyces cerevisiae TSC11/AVO3 is an essential gene encoding one component of TORC2, a multi-protein complex of yeast Tor2p that also contains Lst8p, Avo1p, and Avo2p. Despite the proven physical association among TORC2 components, little is known about the functional linkage or cellular pathways these proteins act in. Here, we present genetic data linking the function of TSC11 to the regulation of cell integrity. Mutants carrying temperature-sensitive (ts) alleles in different regions of TSC11 displayed cell wall defects, evidenced by characteristic osmotic stabilizer-remediable cell lysis, susceptibility to trypan blue staining, and sensitivity to cell wall-digesting enzymes. Dosage suppression analysis identified different groups of genes in rescuing phenotypes of different tsc11ts mutants. AVO1 suppressed one class of mutants, whereas active PKC1, AVO2, and SLM1 partially rescued another. Our findings demonstrate functional connections among TORC2 components and we speculate that Tsc11p exerts its function via a Pkc1p-independent mechanism mediated through Avo1p, and a Pkc1p-dependent mechanism mediated through Avo2p and Slm1p.",

keywords = "Actin, Cell wall, Multicopy suppressor, Protein kinase C, Target of rapamycin",

author = "Ho, {Hsiang Ling} and Shiau, {Yu Shih} and Chen, {Mei Yu}",

note = "Funding Information: Acknowledgements We thank Dr. M.N. Hall for providing plasmids expressing components of the PKC/MAPK pathway. We are very grateful to Dr. J.-J. Lin for providing the S. cerevisiae genomic library and critically reading the manuscript. This work was supported by the Program for Promoting Academic Excellence of Universities (grant 89-B-FA22-2-4) from the Ministry of Education, Taiwan, by grants NSC87-2316-B-010-015, NSC88-2314-B-010-071, and NSC91-2320-B-010-082 from the National Science Council, Taiwan, and by grants NHRI-EX91-8916SC, NHRI-EX92-9230SI, and NHRI-EX93-9230SI from the National Health Research Institutes, Taiwan, to M.-Y.C.",

year = "2005",

month = may,

doi = "10.1007/s00294-005-0570-8",

language = "English",

volume = "47",

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journal = "Current Genetics",

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TY - JOUR

T1 - Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex

AU - Ho, Hsiang Ling

AU - Shiau, Yu Shih

AU - Chen, Mei Yu

N1 - Funding Information: Acknowledgements We thank Dr. M.N. Hall for providing plasmids expressing components of the PKC/MAPK pathway. We are very grateful to Dr. J.-J. Lin for providing the S. cerevisiae genomic library and critically reading the manuscript. This work was supported by the Program for Promoting Academic Excellence of Universities (grant 89-B-FA22-2-4) from the Ministry of Education, Taiwan, by grants NSC87-2316-B-010-015, NSC88-2314-B-010-071, and NSC91-2320-B-010-082 from the National Science Council, Taiwan, and by grants NHRI-EX91-8916SC, NHRI-EX92-9230SI, and NHRI-EX93-9230SI from the National Health Research Institutes, Taiwan, to M.-Y.C.

PY - 2005/5

Y1 - 2005/5

N2 - Saccharomyces cerevisiae TSC11/AVO3 is an essential gene encoding one component of TORC2, a multi-protein complex of yeast Tor2p that also contains Lst8p, Avo1p, and Avo2p. Despite the proven physical association among TORC2 components, little is known about the functional linkage or cellular pathways these proteins act in. Here, we present genetic data linking the function of TSC11 to the regulation of cell integrity. Mutants carrying temperature-sensitive (ts) alleles in different regions of TSC11 displayed cell wall defects, evidenced by characteristic osmotic stabilizer-remediable cell lysis, susceptibility to trypan blue staining, and sensitivity to cell wall-digesting enzymes. Dosage suppression analysis identified different groups of genes in rescuing phenotypes of different tsc11ts mutants. AVO1 suppressed one class of mutants, whereas active PKC1, AVO2, and SLM1 partially rescued another. Our findings demonstrate functional connections among TORC2 components and we speculate that Tsc11p exerts its function via a Pkc1p-independent mechanism mediated through Avo1p, and a Pkc1p-dependent mechanism mediated through Avo2p and Slm1p.

AB - Saccharomyces cerevisiae TSC11/AVO3 is an essential gene encoding one component of TORC2, a multi-protein complex of yeast Tor2p that also contains Lst8p, Avo1p, and Avo2p. Despite the proven physical association among TORC2 components, little is known about the functional linkage or cellular pathways these proteins act in. Here, we present genetic data linking the function of TSC11 to the regulation of cell integrity. Mutants carrying temperature-sensitive (ts) alleles in different regions of TSC11 displayed cell wall defects, evidenced by characteristic osmotic stabilizer-remediable cell lysis, susceptibility to trypan blue staining, and sensitivity to cell wall-digesting enzymes. Dosage suppression analysis identified different groups of genes in rescuing phenotypes of different tsc11ts mutants. AVO1 suppressed one class of mutants, whereas active PKC1, AVO2, and SLM1 partially rescued another. Our findings demonstrate functional connections among TORC2 components and we speculate that Tsc11p exerts its function via a Pkc1p-independent mechanism mediated through Avo1p, and a Pkc1p-dependent mechanism mediated through Avo2p and Slm1p.

KW - Actin

KW - Cell wall

KW - Multicopy suppressor

KW - Protein kinase C

KW - Target of rapamycin

UR - http://www.scopus.com/inward/record.url?scp=19444376122&partnerID=8YFLogxK

U2 - 10.1007/s00294-005-0570-8

DO - 10.1007/s00294-005-0570-8

M3 - Article

C2 - 15809876

AN - SCOPUS:19444376122

SN - 0172-8083

VL - 47

SP - 273

EP - 288

JO - Current Genetics

JF - Current Genetics

IS - 5

ER -

Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex

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Keywords

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