Risk stratification of heart failure from one drop of blood using hand-held biosensor for BNP detection

Indu Sarangadharan, Shin Li Wang, Tse Yu Tai, Anil Kumar Pulikkathodi, Chen Pin Hsu, Hui Hua Kenny Chiang, Lawrence Yu Min Liu, Yu Lin Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Continued risk assessment by evaluating cardiac biomarkers in healthy and unhealthy individuals can lower the mortality rate of cardiovascular diseases (CVDs). In this research, we have developed a hand-held biosensor system to rapidly screen for brain natriuretic peptide (BNP) from a single drop of whole blood. The sensor methodology is based on extended gate design of electrical double layer (EDL) field effect transistor (FET), that can directly detect BNP in whole blood, without extensive sample pre-treatments, thereby eliminating the limitations of charge screening in high ionic strength solutions. A simple sensor array chip is fabricated to integrate with the MOSFET sensor system. Sensing characteristics are elucidated using purified BNP samples in 1 × PBS (with 4% BSA), spiked BNP samples in whole blood and clinical whole blood samples. The blood cells can be gravitationally separated without the use of any external actuation. The sensor exhibits very high sensitivity over wide dynamic range of detection. The sensing characteristics are not adversely affected by the presence of background proteins or blood cells, even without gravitational blood cell separation. Thus, the biosensor system can allow users to perform rapid whole blood diagnostics with minimal user protocols, in 5 min. The features of high sensitivity, cost-effectiveness and convenience of usage empower this technology to revolutionize the mobile diagnostics and healthcare industry.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalBiosensors and Bioelectronics
Volume107
DOIs
StatePublished - 1 Jun 2018

Keywords

  • BNP
  • Extended gate EDL FET biosensor
  • Hand-held device
  • Heart failure
  • Whole blood diagnostics

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