Regulation of thymocyte positive selection and motility by GIT2

Hyewon Phee, Ivan Dzhagalov, Marianne Mollenauer, Yana Wang, Darrell J. Irvine, Ellen Robey, Arthur Weiss*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Thymocytes are highly motile cells that migrate under the influence of chemokines in distinct thymic compartments as they mature. The motility of thymocytes is tightly regulated; however, the molecular mechanisms that control thymocyte motility are not well understood. Here we report that G protein-coupled receptor kinase-interactor 2 (GIT2) was required for efficient positive selection. Notably, Git2-/- double-positive thymocytes showed greater activation of the small GTPase Rac, actin polymerization and migration toward the chemokines CXCL12 (SDF-1) and CCL25 in vitro. By two-photon laser-scanning microscopy, we found that the scanning activity of Git2 -/- thymocytes was compromised in the thymic cortex, which suggests GIT2 has a key role in regulating the chemokine-mediated motility of double-positive thymocytes.

Original languageEnglish
Pages (from-to)503-511
Number of pages9
JournalNature Immunology
Issue number6
StatePublished - Jun 2010


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