Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

Meng Zhe Tsai; Chan Te Hsiung; Yang Chen; Cheng-Sheng Huang; Hsin-Yun Hsu; Pei Ying Hsieh

doi:10.1039/c7an01374d

Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

Meng Zhe Tsai, Chan Te Hsiung, Yang Chen, Cheng-Sheng Huang^*, Hsin-Yun Hsu, Pei Ying Hsieh

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU^-1, which supports a limit of detection of 3.2 ng mL^-1 for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.

Original language	English
Pages (from-to)	503-510
Number of pages	8
Journal	Analyst
Volume	143
Issue number	2
DOIs	https://doi.org/10.1039/c7an01374d
State	Published - 19 Dec 2017

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title = "Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor",

abstract = "We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU-1, which supports a limit of detection of 3.2 ng mL-1 for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.",

author = "Tsai, {Meng Zhe} and Hsiung, {Chan Te} and Yang Chen and Cheng-Sheng Huang and Hsin-Yun Hsu and Hsieh, {Pei Ying}",

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AU - Tsai, Meng Zhe

AU - Hsiung, Chan Te

AU - Chen, Yang

AU - Huang, Cheng-Sheng

AU - Hsu, Hsin-Yun

AU - Hsieh, Pei Ying

PY - 2017/12/19

Y1 - 2017/12/19

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AB - We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU-1, which supports a limit of detection of 3.2 ng mL-1 for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.

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Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

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