Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent

Wen Liang Lo; Jian Yun Lai; Stephen E. Feinberg; Kenji Izumi; Shou Yen Kao; Che Shoa Chang; Alan Lin; Huihua Kenny Chiang

doi:10.1002/jrs.2688

Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent

Wen Liang Lo, Jian Yun Lai, Stephen E. Feinberg, Kenji Izumi, Shou Yen Kao, Che Shoa Chang, Alan Lin, Huihua Kenny Chiang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

To ensure quality control and assurance in tissue engineering, noninvasive, real-time and aseptic evaluation of cell-based devices is required before product release. In this study, Raman spectroscopy was applied to monitor the cellular activities of an oral mucosa equivalent (EVPOME) produced ex vivo from cultured autogenous oral keratinocytes and acellular dermis-AlloDerm. Raman spectra showed a positive correlation of the peak area ratio of amide I (1655 cm^-1)/phenylalanine (1004 cm^-1) with a negative linear regression (R² > 0.95) according to the number of cultured days, especially on the 14thand 21st day. This work demonstrates the successful application of Raman spectroscopy for quantitatively monitoring and evaluating the maturity of EVPOME.

Original language	English
Pages (from-to)	174-178
Number of pages	5
Journal	Journal of Raman Spectroscopy
Volume	42
Issue number	2
DOIs	https://doi.org/10.1002/jrs.2688
State	Published - Feb 2011

Keywords

Raman spectroscopy
ex vivo produced oral mucosal equivalent
tissue engineering

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10.1002/jrs.2688

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abstract = "To ensure quality control and assurance in tissue engineering, noninvasive, real-time and aseptic evaluation of cell-based devices is required before product release. In this study, Raman spectroscopy was applied to monitor the cellular activities of an oral mucosa equivalent (EVPOME) produced ex vivo from cultured autogenous oral keratinocytes and acellular dermis-AlloDerm. Raman spectra showed a positive correlation of the peak area ratio of amide I (1655 cm-1)/phenylalanine (1004 cm-1) with a negative linear regression (R2 > 0.95) according to the number of cultured days, especially on the 14thand 21st day. This work demonstrates the successful application of Raman spectroscopy for quantitatively monitoring and evaluating the maturity of EVPOME.",

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AU - Lo, Wen Liang

AU - Lai, Jian Yun

AU - Feinberg, Stephen E.

AU - Izumi, Kenji

AU - Kao, Shou Yen

AU - Chang, Che Shoa

AU - Lin, Alan

AU - Chiang, Huihua Kenny

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AB - To ensure quality control and assurance in tissue engineering, noninvasive, real-time and aseptic evaluation of cell-based devices is required before product release. In this study, Raman spectroscopy was applied to monitor the cellular activities of an oral mucosa equivalent (EVPOME) produced ex vivo from cultured autogenous oral keratinocytes and acellular dermis-AlloDerm. Raman spectra showed a positive correlation of the peak area ratio of amide I (1655 cm-1)/phenylalanine (1004 cm-1) with a negative linear regression (R2 > 0.95) according to the number of cultured days, especially on the 14thand 21st day. This work demonstrates the successful application of Raman spectroscopy for quantitatively monitoring and evaluating the maturity of EVPOME.

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Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent

Abstract

Keywords

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