Pyridoxal 5′-phosphate binding in lysine-modified PAMAM dendrimers: A biomimetic approach

Kuang Chan Hsien; Hui Ting Chen; Yi Chen Chen; Yeh Long Chen; Chi Yu Lu; Chai Lin Kao

doi:10.1021/ol901299e

Pyridoxal 5′-phosphate binding in lysine-modified PAMAM dendrimers: A biomimetic approach

Kuang Chan Hsien^*, Hui Ting Chen, Yi Chen Chen, Yeh Long Chen, Chi Yu Lu, Chai Lin Kao

^*Corresponding author for this work

Faculty of Pharmacy

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Image Persented (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)_x (2, APO = aminopropanol, Phe = phenylalanine, Lys = lysine) were prepared and used in a binding study with pyridoxal 5′-phosphate. The results revealed a positive dendritic effect, and binding ability was found to vary with the environment. (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)_x (2) demonstrated better binding ability at higher pH, and protonation of lysine was considered to affect binding. The strongest binding affinity was K_b = 254.3 mM ^-1 at pH 9, which was shown by (G:7)-dendri-PAMAM-(APO-Phe-Lys) ₄₉₀ (2e).

Original language	English
Pages (from-to)	3526-3529
Number of pages	4
Journal	Organic Letters
Volume	11
Issue number	16
DOIs	https://doi.org/10.1021/ol901299e
State	Published - 20 Aug 2009

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title = "Pyridoxal 5′-phosphate binding in lysine-modified PAMAM dendrimers: A biomimetic approach",

abstract = "Image Persented (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)x (2, APO = aminopropanol, Phe = phenylalanine, Lys = lysine) were prepared and used in a binding study with pyridoxal 5′-phosphate. The results revealed a positive dendritic effect, and binding ability was found to vary with the environment. (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)x (2) demonstrated better binding ability at higher pH, and protonation of lysine was considered to affect binding. The strongest binding affinity was Kb = 254.3 mM -1 at pH 9, which was shown by (G:7)-dendri-PAMAM-(APO-Phe-Lys) 490 (2e).",

author = "Hsien, {Kuang Chan} and Chen, {Hui Ting} and Chen, {Yi Chen} and Chen, {Yeh Long} and Lu, {Chi Yu} and Kao, {Chai Lin}",

year = "2009",

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doi = "10.1021/ol901299e",

language = "English",

volume = "11",

pages = "3526--3529",

journal = "Organic Letters",

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publisher = "American Chemical Society",

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TY - JOUR

T1 - Pyridoxal 5′-phosphate binding in lysine-modified PAMAM dendrimers

T2 - A biomimetic approach

AU - Hsien, Kuang Chan

AU - Chen, Hui Ting

AU - Chen, Yi Chen

AU - Chen, Yeh Long

AU - Lu, Chi Yu

AU - Kao, Chai Lin

PY - 2009/8/20

Y1 - 2009/8/20

N2 - Image Persented (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)x (2, APO = aminopropanol, Phe = phenylalanine, Lys = lysine) were prepared and used in a binding study with pyridoxal 5′-phosphate. The results revealed a positive dendritic effect, and binding ability was found to vary with the environment. (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)x (2) demonstrated better binding ability at higher pH, and protonation of lysine was considered to affect binding. The strongest binding affinity was Kb = 254.3 mM -1 at pH 9, which was shown by (G:7)-dendri-PAMAM-(APO-Phe-Lys) 490 (2e).

AB - Image Persented (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)x (2, APO = aminopropanol, Phe = phenylalanine, Lys = lysine) were prepared and used in a binding study with pyridoxal 5′-phosphate. The results revealed a positive dendritic effect, and binding ability was found to vary with the environment. (G:3-7)-dendri-PAMAM-(APO-Phe-Lys)x (2) demonstrated better binding ability at higher pH, and protonation of lysine was considered to affect binding. The strongest binding affinity was Kb = 254.3 mM -1 at pH 9, which was shown by (G:7)-dendri-PAMAM-(APO-Phe-Lys) 490 (2e).

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U2 - 10.1021/ol901299e

DO - 10.1021/ol901299e

M3 - Article

C2 - 19621874

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JO - Organic Letters

JF - Organic Letters

IS - 16

ER -

Pyridoxal 5′-phosphate binding in lysine-modified PAMAM dendrimers: A biomimetic approach

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