Pyrene-Based Fluorescent Probe for “Off-on-Off” Sequential Detection of Cu2+ and CN with HeLa Cells Imaging

Muthaiah Shellaiah, Parthiban Venkatesan, Natesan Thirumalaivasan*, Shu Pao Wu*, Kien Wen Sun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The novel pyrene-appended Schiff base probe L with aggregation-induced emissions (AIE) relevant to an increase in water fractions (0–90%) is synthesized and applied in sequentially detecting Cu2+ and CN. The pyrene-based probe L firstly induces the excimer formation in the presence of Cu2+. However, the process can be reversed by sequentially adding CN, which is demonstrated using the fluorescence “Off-On-Off” response in semi-aqueous media ethanol water (v/v = 7/3) under physiological pH (5 mM HEPES, pH 7.0). The Job’s plot, mass analysis, 1H NMR titrations, and density functional theory (DFT) interrogations confirm the 2:1 stoichiometry of excimer complex L–Cu2+-L*, preferential binding atoms, and CN tuned complex reversibility. Based on the photoluminescence (PL) titration, the association constant of L to Cu2+ is determined as 4.95 × 106 M−1. From standard deviation and linear fittings, the detection limits (LODs) of Cu2+ and CN are estimated as 219 nM and 580 nM, respectively. The practicality of Cu2+ and CN detection is demonstrated using a TLC plate and a blended polymer membrane through which significant color changes under a UV lamp can be monitored. Moreover, utility of the designed probe L towards biological application with low toxicity is demonstrated by detecting Cu2+ and CN inside HeLa cells. The responses of the probe to Cu(II) ions were also verified using living HeLa cells imaging.

Original languageEnglish
Article number115
JournalChemosensors
Volume11
Issue number2
DOIs
StatePublished - Feb 2023

Keywords

  • aggregation-induced emissions
  • biocompatibility
  • cell imaging
  • chemosensor
  • copper ion
  • cyanide ion
  • polymer membrane sensor
  • pyrene derivative sequential detection

Fingerprint

Dive into the research topics of 'Pyrene-Based Fluorescent Probe for “Off-on-Off” Sequential Detection of Cu2+ and CN with HeLa Cells Imaging'. Together they form a unique fingerprint.

Cite this