Pulsed-focused ultrasound enhances boron drug accumulation in a human head and neck cancer xenograft-bearing mouse model

Chun Yi Wu; Pei Chia Chan; Lin Shan Chou; Chi Wei Chang; Feng Yi Yang; Ren Shyan Liu; Shih Hwa Chiou; Yi Wei Chen; Sang Hue Yen; Hsin Ell Wang

doi:10.1007/s11307-013-0675-2

Pulsed-focused ultrasound enhances boron drug accumulation in a human head and neck cancer xenograft-bearing mouse model

Chun Yi Wu, Pei Chia Chan, Lin Shan Chou, Chi Wei Chang, Feng Yi Yang, Ren Shyan Liu, Shih Hwa Chiou, Yi Wei Chen, Sang Hue Yen, Hsin Ell Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Purpose: This study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed-HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using ¹⁸F-FBPA-Fr microPET scans. Procedures: To the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, ¹⁸F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted. Results: Both biodistribution studies and microPET imaging performed after intravenous injection of ¹⁸F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control. Conclusion: This study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.

Original language	English
Pages (from-to)	95-101
Number of pages	7
Journal	Molecular Imaging and Biology
Volume	16
Issue number	1
DOIs	https://doi.org/10.1007/s11307-013-0675-2
State	Published - Feb 2014

Keywords

Boron neutron capture therapy
F-FBPA-Fr
Head and neck cancer
MicroPET
Pulsed high-intensity focused ultrasound

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{86d0027319ee446188e479716f1bd3ad,

title = "Pulsed-focused ultrasound enhances boron drug accumulation in a human head and neck cancer xenograft-bearing mouse model",

abstract = "Purpose: This study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed-HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using 18F-FBPA-Fr microPET scans. Procedures: To the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, 18F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted. Results: Both biodistribution studies and microPET imaging performed after intravenous injection of 18F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control. Conclusion: This study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.",

keywords = "Boron neutron capture therapy, F-FBPA-Fr, Head and neck cancer, MicroPET, Pulsed high-intensity focused ultrasound",

author = "Wu, {Chun Yi} and Chan, {Pei Chia} and Chou, {Lin Shan} and Chang, {Chi Wei} and Yang, {Feng Yi} and Liu, {Ren Shyan} and Chiou, {Shih Hwa} and Chen, {Yi Wei} and Yen, {Sang Hue} and Wang, {Hsin Ell}",

note = "Funding Information: Acknowledgement. The authors thank the financial support from Taipei Veterans General Hospital, Taipei, Taiwan (V100A-045 and VGHUST101-G1-2-3). The authors also appreciate the technical support from the Taiwan Mouse Clinic which is funded by the National Research Program for Biopharmaceuticals (NRPB) at the National Science Council (NSC) of Taiwan.",

year = "2014",

month = feb,

doi = "10.1007/s11307-013-0675-2",

language = "English",

volume = "16",

pages = "95--101",

journal = "Molecular Imaging and Biology",

issn = "1536-1632",

publisher = "Springer New York",

number = "1",

}

TY - JOUR

T1 - Pulsed-focused ultrasound enhances boron drug accumulation in a human head and neck cancer xenograft-bearing mouse model

AU - Wu, Chun Yi

AU - Chan, Pei Chia

AU - Chou, Lin Shan

AU - Chang, Chi Wei

AU - Yang, Feng Yi

AU - Liu, Ren Shyan

AU - Chiou, Shih Hwa

AU - Chen, Yi Wei

AU - Yen, Sang Hue

AU - Wang, Hsin Ell

N1 - Funding Information: Acknowledgement. The authors thank the financial support from Taipei Veterans General Hospital, Taipei, Taiwan (V100A-045 and VGHUST101-G1-2-3). The authors also appreciate the technical support from the Taiwan Mouse Clinic which is funded by the National Research Program for Biopharmaceuticals (NRPB) at the National Science Council (NSC) of Taiwan.

PY - 2014/2

Y1 - 2014/2

N2 - Purpose: This study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed-HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using 18F-FBPA-Fr microPET scans. Procedures: To the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, 18F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted. Results: Both biodistribution studies and microPET imaging performed after intravenous injection of 18F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control. Conclusion: This study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.

AB - Purpose: This study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed-HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using 18F-FBPA-Fr microPET scans. Procedures: To the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, 18F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted. Results: Both biodistribution studies and microPET imaging performed after intravenous injection of 18F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control. Conclusion: This study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.

KW - Boron neutron capture therapy

KW - F-FBPA-Fr

KW - Head and neck cancer

KW - MicroPET

KW - Pulsed high-intensity focused ultrasound

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DO - 10.1007/s11307-013-0675-2

M3 - Article

C2 - 23925592

AN - SCOPUS:84895077306

SN - 1536-1632

VL - 16

SP - 95

EP - 101

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 1

ER -

Pulsed-focused ultrasound enhances boron drug accumulation in a human head and neck cancer xenograft-bearing mouse model

Abstract

Keywords

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