PSPC1 mediates TGF-β1 autocrine signalling and Smad2/3 target switching to promote EMT, stemness and metastasis

Hsi Wen Yeh, En Chi Hsu, Szu Shuo Lee, Yaw Dong Lang, Yuh Charn Lin, Chieh Yu Chang, Suz Yi Lee, De Leung Gu, Jou Ho Shih, Chun Ming Ho, Chian Feng Chen, Chiung Tong Chen, Pang Hsien Tu, Ching Feng Cheng, Ruey Hwa Chen, Ruey Bing Yang, Yuh Shan Jou*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

Activation of metastatic reprogramming is critical for tumour metastasis. However, more detailed knowledge of the underlying mechanism is needed to enable targeted intervention. Here, we show that paraspeckle component 1 (PSPC1), identified in an aberrant 13q12.11 locus, is upregulated and associated with poor survival in patients with cancer. PSPC1 promotes tumorigenesis, epithelial-to-mesenchymal transition (EMT), stemness and metastasis in multiple cell types and in spontaneous mouse cancer models. PSPC1 is the master activator for transcription factors of EMT and stemness and accompanies c-Myc activation to facilitate tumour growth. PSPC1 increases transforming growth factor-β1 (TGF-β1) secretion through an interaction with phosphorylated and nuclear Smad2/3 to potentiate TGF-β1 autocrine signalling. Moreover, PSPC1 acts as a contextual determinant of the TGF-β1 pro-metastatic switch to alter Smad2/3 binding preference from tumour-suppressor to pro-metastatic genes. Having validated the PSPC1-Smads-TGF-β1 axis in various cancers, we conclude that PSPC1 is a master activator of pro-metastatic switches and a potential target for anti-metastasis drugs.

Original languageEnglish
Pages (from-to)479-491
Number of pages13
JournalNature Cell Biology
Volume20
Issue number4
DOIs
StatePublished - 1 Apr 2018

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