Protocol for standardized intrathymic injection in mice

Tsung Lin Tsai; Pei Yuan Tsai; Ivan L. Dzhagalov; Chia Lin Hsu

doi:10.1016/j.xpro.2022.102010

Protocol for standardized intrathymic injection in mice

Tsung Lin Tsai
, Pei Yuan Tsai^*
, Ivan L. Dzhagalov
, Chia Lin Hsu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).¹

Original language	English
Article number	102010
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.102010
State	Published - 17 Mar 2023

Keywords

Developmental Biology
Immunology

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10.1016/j.xpro.2022.102010

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title = "Protocol for standardized intrathymic injection in mice",

abstract = "Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1",

keywords = "Developmental Biology, Immunology",

author = "Tsai, \{Tsung Lin\} and Tsai, \{Pei Yuan\} and Dzhagalov, \{Ivan L.\} and Hsu, \{Chia Lin\}",

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year = "2023",

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doi = "10.1016/j.xpro.2022.102010",

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T1 - Protocol for standardized intrathymic injection in mice

AU - Tsai, Tsung Lin

AU - Tsai, Pei Yuan

AU - Dzhagalov, Ivan L.

AU - Hsu, Chia Lin

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1

AB - Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1

KW - Developmental Biology

KW - Immunology

UR - https://www.scopus.com/pages/publications/85148700634

U2 - 10.1016/j.xpro.2022.102010

DO - 10.1016/j.xpro.2022.102010

M3 - Article

C2 - 36638012

AN - SCOPUS:85148700634

SN - 2666-1667

VL - 4

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 102010

ER -

Protocol for standardized intrathymic injection in mice

Abstract

Keywords

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