Protocol for standardized intrathymic injection in mice

Tsung Lin Tsai; Pei Yuan Tsai; Ivan L. Dzhagalov; Chia-Lin Hsu

doi:10.1016/j.xpro.2022.102010

Protocol for standardized intrathymic injection in mice

Tsung Lin Tsai, Pei Yuan Tsai^*, Ivan L. Dzhagalov, Chia-Lin Hsu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).¹

Original language	English
Article number	102010
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.102010
State	Published - 17 Mar 2023

Keywords

Developmental Biology
Immunology

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10.1016/j.xpro.2022.102010

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title = "Protocol for standardized intrathymic injection in mice",

abstract = "Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1",

keywords = "Developmental Biology, Immunology",

author = "Tsai, {Tsung Lin} and Tsai, {Pei Yuan} and Dzhagalov, {Ivan L.} and Chia-Lin Hsu",

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doi = "10.1016/j.xpro.2022.102010",

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AU - Tsai, Tsung Lin

AU - Tsai, Pei Yuan

AU - Dzhagalov, Ivan L.

AU - Hsu, Chia-Lin

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1

AB - Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1

KW - Developmental Biology

KW - Immunology

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U2 - 10.1016/j.xpro.2022.102010

DO - 10.1016/j.xpro.2022.102010

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VL - 4

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 102010

ER -

Protocol for standardized intrathymic injection in mice

Abstract

Keywords

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