Protocol for standardized intrathymic injection in mice

Tsung Lin Tsai, Pei Yuan Tsai*, Ivan L. Dzhagalov, Chia-Lin Hsu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).1

Original languageEnglish
Article number102010
JournalSTAR Protocols
Issue number1
StatePublished - 17 Mar 2023


  • Developmental Biology
  • Immunology


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