Proteomic analysis of exosomes derived from the aqueous humor of myopia patients

Ching Yao Tsai, Chueh Tan Chen, Chin Hui Lin, Chen Chung Liao, Kate Hua, Chung Hua Hsu, Chian Feng Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Objectives: Myopia is the most common refractive vision disorder. In recent years, several studies have suggested that the alteration of the exosomal protein levels in the aqueous humor (AH) is associated with the development of several eye diseases. Therefore, we aimed to explore the exosomal protein profile of the AH from myopia patients. Methods: Exosomes were isolated from the AH. The quality, concentration, and size distribution of exosomes for each patient were measured using nanoparticle tracking analysis system. Then, the exosomal proteins were purified and digested by trypsin for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Results: There was no significant difference observed between the myopia and control when comparing the concentration and size distribution of exosomes in the AH for each sample. Based on LC-MS/MS analysis, myopia patients had higher and more complex exosomal peptide content. We found two proteins that were common in AH exosomes and eight proteins that were highly expressed in the myopia group. Conclusions: Our results provide pioneering findings for the exploration of the exosomal protein profile in myopia development. Further studies may provide significant information for the diagnosis, clinical treatment, and prognosis of myopia.

Original languageEnglish
Pages (from-to)2023-2029
Number of pages7
JournalInternational Journal of Medical Sciences
Volume18
Issue number9
DOIs
StatePublished - 10 Mar 2021

Keywords

  • Aqueous humor
  • Exosome
  • Myopia

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