Profiles of oral microbiome associated with nasogastric tube feeding

Ding Han Wang, Fa Tzu Tsai, Hsi Feng Tu, Cheng Chieh Yang, Ming Lun Hsu, Lin Jack Huang, Chiu Tzu Lin, Wun Eng Hsu, Yu Cheng Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Background: Dysbiosis of oral microbiome causes chronic diseases including dental caries and periodontitis, which frequently affect older patient populations. Severely disabled individuals with impaired swallowing functions may require nutritional supply via nasogastric (NG) tubes, further impacting their oral condition and possibly microbial composition. However, little is known about the effect of NG tube on oral microbes and its potential ramification. Methods: By using 16S rRNA amplicon sequencing, we characterized the tongue microbiome of 27 patients fed with NG tubes and 26 others fed orally. Results: The microbial compositions of NG-tube and oral-feeding patients were substantially different, with more Gram-negative aerobes enriched in the presence of NG tube. Specifically, NG-tube patients presented more opportunistic pathogens like Pseudomonas and Corynebacterium associated with pneumonia and lower levels of commensal Streptococcus and Veillonella. Co-occurrence analysis further showed an inverse relationship between commensal and pathogenic species. Conclusion: We present a systematic, high-throughput profiling of oral microbiome with regard to long-term NG tube feeding among the older patient population.

Original languageEnglish
Article number2200898
JournalJournal of Oral Microbiology
Issue number1
StatePublished - 2023


  • 16S rRNA
  • amplicon sequence variant
  • aspiration pneumonia
  • nasogastric tube
  • Oral microbiome


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