Probing the interior of synaptic vesicles with internalized nanoparticles

Jennifer C. Gadd, Kristi L. Budzinski, Yang-Hsiang Chan, Fangmao Ye, Daniel T. Chiu*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Synaptic vesicles are subcellular organelles that are found in the synaptic bouton and are responsible for the propagation of signals between neurons. Synaptic vesicles undergo endo- and exocytosis with the neuronal membrane to load and release neurotransmitters. Here we discuss how we utilize this property to load nanoparticles as a means of probing the interior of synaptic vesicles. To probe the intravesicular region of synaptic vesicles, we have developed a highly sensitive pH-sensing polymer dot. We feel the robust nature of the pH-sensing polymer dot will provide insight into the dynamics of proton loading into synaptic vesicles.

Original languageEnglish
Title of host publicationColloidal Nanocrystals for Biomedical Applications VII
DOIs
StatePublished - 2012
EventColloidal Nanocrystals for Biomedical Applications VII - San Francisco, CA, United States
Duration: 21 Jan 201222 Jan 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8232
ISSN (Print)1605-7422

Conference

ConferenceColloidal Nanocrystals for Biomedical Applications VII
Country/TerritoryUnited States
CitySan Francisco, CA
Period21/01/1222/01/12

Keywords

  • Förster resonance energy transfer (FRET)
  • PH sensor
  • Polymer dots
  • Single molecule
  • Synaptic vesicles
  • Synaptosome

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