TY - JOUR
T1 - Probing algogenic organic matter (AOM) by size-exclusion chromatography to predict AOM-derived disinfection by-product formation
AU - Hua, Lap Cuong
AU - Lin, Jr Lin
AU - Chao, Shu Ju
AU - Huang, Ch-Hpin
PY - 2018/12/15
Y1 - 2018/12/15
N2 - High-performance size exclusion chromatography (HPSEC) coupled with peak-fitting technique was used to probe molecular weight (MW) properties of algogenic organic matter (AOM). The qualitative and quantitative MW information derived was used to predict AOM-derived disinfection by-product (DBP) formation. We resolved overlapping HPSEC chromatograms of all AOM samples into six major peaks with R2 > 0.996. This study gave significant insight into the HPSEC profiles of AOM, in which resolved peaks A and B (biopolymers) and peak C (humic substances) showed a strong correlation with the formation of carbonaceous-DBPs (C-DBPs). This likely resulted from the abundance of aromatic structures and conjugated C[dbnd]C double bonds in their chemical nature. Our results also indicated the importance of algal cells, including intra-cellular and cell-bound organic matter, over extra-cellular organic matter as precursors to C-DBP formation. The application of the information extracted from HPSEC profiles associated with the fluorescent components of AOM showed great improvements in the predictability of THMs, HAAs, and C-DBPs with R2 > 0.7 and p < 0.05. The outcome of this study will significantly benefit effective control of AOM-derived DBP formation by the chlorination of eutrophic waters.
AB - High-performance size exclusion chromatography (HPSEC) coupled with peak-fitting technique was used to probe molecular weight (MW) properties of algogenic organic matter (AOM). The qualitative and quantitative MW information derived was used to predict AOM-derived disinfection by-product (DBP) formation. We resolved overlapping HPSEC chromatograms of all AOM samples into six major peaks with R2 > 0.996. This study gave significant insight into the HPSEC profiles of AOM, in which resolved peaks A and B (biopolymers) and peak C (humic substances) showed a strong correlation with the formation of carbonaceous-DBPs (C-DBPs). This likely resulted from the abundance of aromatic structures and conjugated C[dbnd]C double bonds in their chemical nature. Our results also indicated the importance of algal cells, including intra-cellular and cell-bound organic matter, over extra-cellular organic matter as precursors to C-DBP formation. The application of the information extracted from HPSEC profiles associated with the fluorescent components of AOM showed great improvements in the predictability of THMs, HAAs, and C-DBPs with R2 > 0.7 and p < 0.05. The outcome of this study will significantly benefit effective control of AOM-derived DBP formation by the chlorination of eutrophic waters.
KW - Algogenic organic matter
KW - Chlorination
KW - Disinfection byproducts
KW - Eutrophication
KW - Size exclusion chromatography
UR - http://www.scopus.com/inward/record.url?scp=85049793005&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2018.07.100
DO - 10.1016/j.scitotenv.2018.07.100
M3 - Article
C2 - 30015120
AN - SCOPUS:85049793005
SN - 0048-9697
VL - 645
SP - 71
EP - 78
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -