Potential of faecal RNA in diagnosing colorectal cancer

Shung Haur Yang, Chih Cheng Chien, Chan Wei Chen, Shih Yun Li, Chi Jung Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Early diagnosis for colorectal cancer (CRC) by monitoring of gastrointestinal epithelial cells is a possible direct approach. Although human faeces form a heterogeneous mixture of gastrointestinal mucosal epithelial cells and other materials, we have developed a method to purify total RNA from human stool samples. The gene for faecal cytokeratin 19 (CK19) was highly expressed in stools from patients with metastatic CRC, but not from patients with non-metastatic CRC or from normal individuals. Thus, purified faecal RNA can be used for the detection of differentially expressed genes. This technique may help identifying meaningful faecal RNA markers for the non-invasive screening of patients with CRC.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalCancer Letters
Volume226
Issue number1
DOIs
StatePublished - 8 Aug 2005

Keywords

  • Colorectal cancer
  • Cytokeratin 19
  • Differential expressed genes
  • Faecal total RNA

Fingerprint

Dive into the research topics of 'Potential of faecal RNA in diagnosing colorectal cancer'. Together they form a unique fingerprint.

Cite this