Physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus

Chi Ju Chen; Douglas J. Leisy; Suzanne M. Thiem

doi:10.1099/0022-1317-77-2-167

Physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus

Chi Ju Chen, Douglas J. Leisy, Suzanne M. Thiem^*

^*Corresponding author for this work

Institute of Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus (AfMNPV) DNA was constructed for restriction endonucleases EcoRI, HindIII, PstI and XhoI. The genome size was estimated to be 130 kbp. Ordering of the restriction fragments was accomplished by cross-blot hybridization, double digestion and DNA-DNA hybridization. The polyhedrin gene and homologous repeat (hr) regions were located by hybridization to the Autographa californica MNPV (AcMNPV) polyhedrin gene and hr4, respectively. Restriction pattern comparison and Southern blot analysis suggest that AfMNPV is closely related to AcMNPV.

Original language	English
Pages (from-to)	167-171
Number of pages	5
Journal	Journal of General Virology
Volume	77
Issue number	2
DOIs	https://doi.org/10.1099/0022-1317-77-2-167
State	Published - 1996

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title = "Physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus",

abstract = "A physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus (AfMNPV) DNA was constructed for restriction endonucleases EcoRI, HindIII, PstI and XhoI. The genome size was estimated to be 130 kbp. Ordering of the restriction fragments was accomplished by cross-blot hybridization, double digestion and DNA-DNA hybridization. The polyhedrin gene and homologous repeat (hr) regions were located by hybridization to the Autographa californica MNPV (AcMNPV) polyhedrin gene and hr4, respectively. Restriction pattern comparison and Southern blot analysis suggest that AfMNPV is closely related to AcMNPV.",

author = "Chen, {Chi Ju} and Leisy, {Douglas J.} and Thiem, {Suzanne M.}",

year = "1996",

doi = "10.1099/0022-1317-77-2-167",

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AU - Leisy, Douglas J.

AU - Thiem, Suzanne M.

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Physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus

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