Abstract
Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain.
Original language | English |
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Pages (from-to) | 85-91 |
Number of pages | 7 |
Journal | Optics Communications |
Volume | 201 |
Issue number | 1-3 |
DOIs | |
State | Published - 1 Jan 2002 |
Keywords
- Chloroplasts
- Micro-spectroscopy
- Multi-phpton excition
- Photobleaching