Photobleaching under single photon and multi-photon excitation: Chloroplasts in protoplasts from Arabidopsis thaliana

Fu Jen Kao*, Yi Min Wang, Jian Cheng Chen, Ping Chin Cheng, Rung Wu Chen, Bai Ling Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalOptics Communications
Volume201
Issue number1-3
DOIs
StatePublished - 1 Jan 2002

Keywords

  • Chloroplasts
  • Micro-spectroscopy
  • Multi-phpton excition
  • Photobleaching

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