Phostensin caps to the pointed end of actin filaments and modulates actin dynamics

Ning Sheng Lai; Tzu Fan Wang; Shiu Lan Wang; Chun Yu Chen; Jia yi Yen; Hsien lu Huang; Chin Li; Kuang Yung Huang; Su Qin Liu; Ta Hsien Lin; Hsien bin Huang

doi:10.1016/j.bbrc.2009.07.086

Phostensin caps to the pointed end of actin filaments and modulates actin dynamics

Ning Sheng Lai, Tzu Fan Wang, Shiu Lan Wang, Chun Yu Chen, Jia yi Yen, Hsien lu Huang, Chin Li, Kuang Yung Huang, Su Qin Liu, Ta Hsien Lin^*, Hsien bin Huang

^*Corresponding author for this work

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949, consists of 165 amino acids and is located between HLA-C and HLA-E gene clusters on human chromosome 6. In this current study, we characterized the biochemical functions of phostensin. Actin dynamics assays using gelsolin-actin seeds showed that phostensin decreases the elongation and depolymerization rates of actin filament pointed ends. The feature of phostensin that binds to the pointed ends of actin filaments was observed through fluorescent single filament binding assay. Taken together, our results suggested that phostensin is an actin filament pointed end-capping protein that is capable of modulating actin dynamics.

Original language	English
Pages (from-to)	676-681
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	387
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.07.086
State	Published - 2 Oct 2009

Keywords

Actin filament
KIAA1949
Phostensin
Protein phosphatase 1
Tropomodulin

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10.1016/j.bbrc.2009.07.086

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@article{b4313b375a7d4f1c887868bfad77a6a8,

title = "Phostensin caps to the pointed end of actin filaments and modulates actin dynamics",

abstract = "Phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949, consists of 165 amino acids and is located between HLA-C and HLA-E gene clusters on human chromosome 6. In this current study, we characterized the biochemical functions of phostensin. Actin dynamics assays using gelsolin-actin seeds showed that phostensin decreases the elongation and depolymerization rates of actin filament pointed ends. The feature of phostensin that binds to the pointed ends of actin filaments was observed through fluorescent single filament binding assay. Taken together, our results suggested that phostensin is an actin filament pointed end-capping protein that is capable of modulating actin dynamics.",

keywords = "Actin filament, KIAA1949, Phostensin, Protein phosphatase 1, Tropomodulin",

author = "Lai, {Ning Sheng} and Wang, {Tzu Fan} and Wang, {Shiu Lan} and Chen, {Chun Yu} and Yen, {Jia yi} and Huang, {Hsien lu} and Chin Li and Huang, {Kuang Yung} and Liu, {Su Qin} and Lin, {Ta Hsien} and Huang, {Hsien bin}",

note = "Funding Information: This work was support by grants from the National Science Council, ROC (NSC 96-2320-B-194-005-MY3, NSC 95-2320-B-194-005 and NSC 92-2311-B-010-003), the Taipei Veterans General Hospital, Taiwan, ROC (V95S4-007 and V96S4-002) and the DaLin Tzu Chi Buddhist Hospital (DTCRD95(2)-16 and DTCRD95(2)-17).",

year = "2009",

month = oct,

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doi = "10.1016/j.bbrc.2009.07.086",

language = "English",

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pages = "676--681",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Phostensin caps to the pointed end of actin filaments and modulates actin dynamics

AU - Lai, Ning Sheng

AU - Wang, Tzu Fan

AU - Wang, Shiu Lan

AU - Chen, Chun Yu

AU - Yen, Jia yi

AU - Huang, Hsien lu

AU - Li, Chin

AU - Huang, Kuang Yung

AU - Liu, Su Qin

AU - Lin, Ta Hsien

AU - Huang, Hsien bin

N1 - Funding Information: This work was support by grants from the National Science Council, ROC (NSC 96-2320-B-194-005-MY3, NSC 95-2320-B-194-005 and NSC 92-2311-B-010-003), the Taipei Veterans General Hospital, Taiwan, ROC (V95S4-007 and V96S4-002) and the DaLin Tzu Chi Buddhist Hospital (DTCRD95(2)-16 and DTCRD95(2)-17).

PY - 2009/10/2

Y1 - 2009/10/2

N2 - Phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949, consists of 165 amino acids and is located between HLA-C and HLA-E gene clusters on human chromosome 6. In this current study, we characterized the biochemical functions of phostensin. Actin dynamics assays using gelsolin-actin seeds showed that phostensin decreases the elongation and depolymerization rates of actin filament pointed ends. The feature of phostensin that binds to the pointed ends of actin filaments was observed through fluorescent single filament binding assay. Taken together, our results suggested that phostensin is an actin filament pointed end-capping protein that is capable of modulating actin dynamics.

AB - Phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949, consists of 165 amino acids and is located between HLA-C and HLA-E gene clusters on human chromosome 6. In this current study, we characterized the biochemical functions of phostensin. Actin dynamics assays using gelsolin-actin seeds showed that phostensin decreases the elongation and depolymerization rates of actin filament pointed ends. The feature of phostensin that binds to the pointed ends of actin filaments was observed through fluorescent single filament binding assay. Taken together, our results suggested that phostensin is an actin filament pointed end-capping protein that is capable of modulating actin dynamics.

KW - Actin filament

KW - KIAA1949

KW - Phostensin

KW - Protein phosphatase 1

KW - Tropomodulin

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DO - 10.1016/j.bbrc.2009.07.086

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SN - 0006-291X

VL - 387

SP - 676

EP - 681

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Phostensin caps to the pointed end of actin filaments and modulates actin dynamics

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