Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) upregulated E-cadherin expression in HepG2 cells

Hui Ju Lee, Yeu Su, Wing Yiu Lui, Gar Yang Chau, Pen Hui Yin, Hsin Chen Lee*, Chin Wen Chi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), a highly inducible transcriptional coactivator regulating energy homeostasis, is down-regulated in hepatoma tissues. To dissect its role in hepato-tumorigenesis, Ingenuity® Pathway Analysis was applied to construct pathways affected by PGC-1α upregulation in HepG2 hepatoma cells based on our microarray data. Interestingly, migration of these cells was markedly diminished by PGC-1α overexpression in consistency with Ingenuity® results. Moreover, a deduced expression increase of E-cadherin was also observed in PGC-1α-overexpressing HepG2 cells. Finally, transient transfection and chromatin-immunoprecipitation assays suggested that increased histone acetylation might be responsible for PGC-1α-mediated transactivation of a minimal E-cadherin promoter.

Original languageEnglish
Pages (from-to)627-634
Number of pages8
JournalFEBS Letters
Volume582
Issue number5
DOIs
StatePublished - 5 Mar 2008

Keywords

  • E-cadherin
  • Hepatocellular carcinoma
  • Histone modification
  • Migration
  • PGC-1α
  • Transcription

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