P-cadherin and e-cadherin are co-expressed in mdck cells

Jiahn Chun Wu, Christopher W. Gregory, Robert M. Dephilip

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The Madin-Darby canine kidney (MDCK) cell line is a valuable model of epithelial cell function and the role of E(epithelial)-cadherin in MDCK cell activity is well-appreciated. Northern and immunoblot analyses were used here to demonstrate that, in addition to E-cadherin, MDCK cell cultures also express P(placental)-cadherin. Immunocytochemistry was then used to compare the localization and detergent extractability of P- and E-cadherin. While both cadherins were found at areas of cell-cell contact, the relative ease with which P-cadherin could be extracted suggests a weaker anchorage to the actin cytoskeleton than has been demonstrated for E-cadherin, and may correspond to less permanent cell-cell interactions than those attributed to E-cadherin. The co-expression of different cadherins by MDCK cells has not been fully explored and may influence interpretations of MDCK cell function that previously considered only the activity of E-cadherin.

Original languageEnglish
Pages (from-to)1329-1335
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - 30 Sep 1993


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