Oxygenated water inhibits adipogenesis and attenuates hepatic steatosis in high-fat diet-induced obese mice

Yuh Jen Cheng, Chao Chi Liu, Fang Yeh Chu, Ching Ping Yang, Chiao Wan Hsiao, Cheng Wei Chuang, Ming Yuh Shiau, Hsueh Te Lee, Jen Ning Tsai, Yih Hsin Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The expansion of adipose tissue mass is the primary characteristic of the process of becoming obesity, which causes chronic adipose inflammation and is closely associated with type 2 diabetes mellitus (T2DM). Adipocyte hypertrophy restricts oxygen availability, leading to microenvironmental hypoxia and adipose dysfunction. This study aimed at investigating the effects of oxygenated water (OW) on adipocyte differentiation (adipogenesis) and the metabolic function of mature adipocytes. The effects of OW on adipogenesis and the metabolic function of mature adipocytes were examined. Meanwhile, the in vivo metabolic effects of long-term OW consumption on diet-induced obesity (DIO) mice were investigated. OW inhibited adipogenesis and lipid accumulation through down-regulating critical adipogenic transcription factors and lipogenic enzymes. While body weight, blood and adipose parameters were not significantly improved by long-term OW consumption, transient circulatory triglyceride-lowering and glucose toleranceimproving effects were identified. Notably, hepatic lipid contents were significantly reduced, indicating that the DIO-induced hepatic steatosis was attenuated, despite no improvements in fibrosis and lipid contents in adipose tissue being observed in the OW-drinking DIO mice. The study provides evidence regarding OW’s effects on adipogenesis and mature adipocytes, and the corresponding molecular mechanisms. OW exhibits transient triglyceride-lowering and glucose tolerance-improving activity as well as hepatic steatosis-attenuating functions.

Original languageEnglish
Article number5493
Pages (from-to)1-18
Number of pages18
JournalInternational Journal Of Molecular Sciences
Issue number15
StatePublished - 1 Aug 2020


  • Adipocytes
  • Adipogenesis
  • Hepatic steatosis
  • Lipid metabolism
  • Oxygenated water


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