Abstract
A novel optical heterodyne surface plasmon resonance (SPR) biosensor using Zeeman laser is proposed. There are two surface plasma waves (SPWs) being excited by two correlated P polarized waves in an SPR device of Kretschmann configuration. The two reflected P waves are optically heterodyned so that the magnitude of the heterodyned signal is proportional to the multiplication of two attenuated reflected P waves. Then the detection sensitivity and the dynamical range based on this amplitude sensitive method are enhanced. In the experiment, the kinetics between mouse IgG and anti-mouse IgG is obtained according to the sensograms of different concentrations of anti-mouse IgG. The detection sensitivity corresponding to 0.2 nM is achieved. In addition, a concentration of 5 ng/ml of protein G interacting with mouse IgG is measured successfully.
| Original language | English |
|---|---|
| Pages (from-to) | 149-156 |
| Number of pages | 8 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 4957 |
| DOIs | |
| State | Published - 2003 |
| Event | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Fibers and Sensors for Medical Applications III - San Jose, CA, United States Duration: 25 Jan 2003 → 28 Jan 2003 |
Keywords
- Biosensor
- Optical heterodyne
- Surface plasma wave (SPW)
- Surface plasmon resonance (SPR)