On the adsorption kinetics of bovine serum albumin at the air–water interface

Thu Thi-Yen Le, Siam Hussain, Ruey Yug Tsay, Shi Yow Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The adsorption kinetics of bovine serum albumin (BSA) at an air–water interface was investigated by experimentally measuring and theoretically modeling the dynamic surface tension (ST) data. A pendant bubble tensiometer was utilized for two ST measurements: (i) the adsorption onto a freshly created bubble surface and (ii) the bubble expansion/compression at the latter stage. The dynamic ST data, during the early stage of BSA adsorption, were best-fitted with the short-time approximation equation (diffusion-control) and the non-asymptomatic short-time formalism [mixed-control, Moorkanikkara and Blankschtein, JCIS, 296, 442–457 (2006)]. The fitting showed that the non-asymptotic short-time formalism could well predict the dynamic ST data; thereby, implying that the adsorption of BSA onto a clean air–water interface was mixed-controlled. A new approach for estimating the maximum surface concentration (Γ) and the intermolecular interaction amongst the adsorbed BSA molecules (K) was trialed. The relaxation of ST during a surface expansion was best-fitted with the Langmuir/Frumkin equation of state. A reasonably good fitting between the dynamic ST data and the theoretical ST profiles indicated that this approach could be used to estimate the Γ and K for BSA without requiring the equilibrium ST data.

Original languageEnglish
Article number118813
JournalJournal of Molecular Liquids
Volume353
DOIs
StatePublished - 1 May 2022

Keywords

  • Adsorption kinetics
  • Bovine serum albumin
  • Diffusion-control
  • Dynamic surface tension
  • Mixed-control
  • Surface perturbation

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