TY - GEN
T1 - On-chip RBC deformability checker embedded with vision analyzer
AU - Kaneko, Makoto
AU - Ishida, Takuto
AU - Tsai, Chia-Hung
AU - Ito, Hiroaki
AU - Chimura, Misato
AU - Taniguchi, Tatsunori
AU - Ohtani, Tomohito
AU - Sakata, Yasushi
PY - 2017/8/23
Y1 - 2017/8/23
N2 - This paper discusses an on-chip Red Blood Cell (RBC) deformability checker with vision analyzer. The system is composed of a PDMS chip including three microfluidic channels, a microscope for appropriately enlarging RBC, and a high-speed vision for observing both the cell size and behaviors in the channel, respectively. We particularly focus on how to analyze the deformability of single cells by the proposed vision analyzer. The analyzer can detect the diameter and velocity of each cell inside and outside the test channel. Based on the information, we can obtain a deformability map which shows the relationship between cell deformation and transit velocity. The results obtained by the proposed vision analyzer are compared to the manual evaluation with a tuning parameter based on gray level control. The comparison results show that the error is less than 5% between vision-based and manual evaluations.
AB - This paper discusses an on-chip Red Blood Cell (RBC) deformability checker with vision analyzer. The system is composed of a PDMS chip including three microfluidic channels, a microscope for appropriately enlarging RBC, and a high-speed vision for observing both the cell size and behaviors in the channel, respectively. We particularly focus on how to analyze the deformability of single cells by the proposed vision analyzer. The analyzer can detect the diameter and velocity of each cell inside and outside the test channel. Based on the information, we can obtain a deformability map which shows the relationship between cell deformation and transit velocity. The results obtained by the proposed vision analyzer are compared to the manual evaluation with a tuning parameter based on gray level control. The comparison results show that the error is less than 5% between vision-based and manual evaluations.
KW - RBC Deformability
KW - Vision analyzer
UR - http://www.scopus.com/inward/record.url?scp=85030329597&partnerID=8YFLogxK
U2 - 10.1109/ICMA.2017.8016126
DO - 10.1109/ICMA.2017.8016126
M3 - Conference contribution
AN - SCOPUS:85030329597
T3 - 2017 IEEE International Conference on Mechatronics and Automation, ICMA 2017
SP - 2005
EP - 2010
BT - 2017 IEEE International Conference on Mechatronics and Automation, ICMA 2017
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 14th IEEE International Conference on Mechatronics and Automation, ICMA 2017
Y2 - 6 August 2017 through 9 August 2017
ER -