NUSAS: Negative pressure driving HEPG2/3T3 cells mixing/gradient co-culture inside U trapper array on rapid multicellular spheroid assembling system

S. M. Yang, C. Y. Fu, S. Y. Tseng, V. P. Srinivasu, S. Shilpa, H. Y. Chang, Long Hsu, C. H. Liu*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This article presents a Negative-pressure-powered U-trapper of Spheroid Assembling System (NUSAS) and four unique features are addressed: (1) Negative pressure powered medium driving, (2) HepG2/3T3 gradient distribution and mixing co-culture, (3) Cell trapping effect via gravity force and liquid flow co-activation, and (4) Large area of U trapper array. The development of in vitro liver device benefits liver toxicology and metabolism.[1,2] However, due to the complexity of human liver tissue, it has been rarely discussed what kind of hepatocyte to fibroblast ratio has nearest performance to a real liver tissue. Here, we culture liver/fibroblast cells with gradient ratio as multicellular assembling spheroids simultaneously to find this ratio. These results based on our efficient approaches give us meaningful foundations to investigate biomedical character of 3D HepG2/3T3 artificial tissue in different mixing cell conditions.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages1077-1080
Number of pages4
DOIs
StatePublished - 7 May 2012
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 29 Jan 20122 Feb 2012

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country/TerritoryFrance
CityParis
Period29/01/122/02/12

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