Novel rhodamine probe for colorimetric and fluorescent detection of Fe³⁺ ions in aqueous media with cellular imaging

A novel rhodamine-pyridine conjugated spectroscopic probe RhP was synthesized and its X-ray single crystalline properties were revealed with tabulation. The RhP displayed a distinct pale-pink colorimetric and “turn-on” fluorescent response to Fe³⁺ in aqueous media [H₂O:DMSO (95:5, v/v)] than that of other interfering ions. During the Fe³⁺ recognition, the absorption (UV–Vis) and photoluminescence (PL) spectral studies revealed new peaks at 561 and 592 nm, respectively. The 1:1 stoichiometry and binding sites were verified by Job's plot, ESI-mass, and ¹H NMR titrations. Subsequently, LOD and binding constant for RhP + Fe³⁺ complex were estimated as 102.3 nM and 6.265 × 10⁴ M⁻¹ from linear fitting and Benesi-Hildebrand plots, correspondingly. Sensor reversibility of RhP + Fe³⁺ by EDTA was demonstrated by UV/PL and TRPL investigations. Moreover, the photoinduced energy transfer mechanism and band gap changes were established from the DFT interrogations. Lastly, cellular imaging studies were carried out to authenticate the real applicability of RhP in Fe³⁺ detection.