TY - JOUR
T1 - Novel rhodamine probe for colorimetric and fluorescent detection of Fe3+ ions in aqueous media with cellular imaging
AU - Shellaiah, Muthaiah
AU - Thirumalaivasan, Natesan
AU - Aazaad, Basheer
AU - Awasthi, Kamlesh
AU - Sun, Kien-Wen
AU - Wu, Shu-Pao
AU - Lin, Ming-Chang
AU - Ohta, Nobuhiro
PY - 2020/12/5
Y1 - 2020/12/5
N2 - A novel rhodamine-pyridine conjugated spectroscopic probe RhP was synthesized and its X-ray single crystalline properties were revealed with tabulation. The RhP displayed a distinct pale-pink colorimetric and “turn-on” fluorescent response to Fe3+ in aqueous media [H2O:DMSO (95:5, v/v)] than that of other interfering ions. During the Fe3+ recognition, the absorption (UV–Vis) and photoluminescence (PL) spectral studies revealed new peaks at 561 and 592 nm, respectively. The 1:1 stoichiometry and binding sites were verified by Job's plot, ESI-mass, and 1H NMR titrations. Subsequently, LOD and binding constant for RhP + Fe3+ complex were estimated as 102.3 nM and 6.265 × 104 M−1 from linear fitting and Benesi-Hildebrand plots, correspondingly. Sensor reversibility of RhP + Fe3+ by EDTA was demonstrated by UV/PL and TRPL investigations. Moreover, the photoinduced energy transfer mechanism and band gap changes were established from the DFT interrogations. Lastly, cellular imaging studies were carried out to authenticate the real applicability of RhP in Fe3+ detection.
AB - A novel rhodamine-pyridine conjugated spectroscopic probe RhP was synthesized and its X-ray single crystalline properties were revealed with tabulation. The RhP displayed a distinct pale-pink colorimetric and “turn-on” fluorescent response to Fe3+ in aqueous media [H2O:DMSO (95:5, v/v)] than that of other interfering ions. During the Fe3+ recognition, the absorption (UV–Vis) and photoluminescence (PL) spectral studies revealed new peaks at 561 and 592 nm, respectively. The 1:1 stoichiometry and binding sites were verified by Job's plot, ESI-mass, and 1H NMR titrations. Subsequently, LOD and binding constant for RhP + Fe3+ complex were estimated as 102.3 nM and 6.265 × 104 M−1 from linear fitting and Benesi-Hildebrand plots, correspondingly. Sensor reversibility of RhP + Fe3+ by EDTA was demonstrated by UV/PL and TRPL investigations. Moreover, the photoinduced energy transfer mechanism and band gap changes were established from the DFT interrogations. Lastly, cellular imaging studies were carried out to authenticate the real applicability of RhP in Fe3+ detection.
KW - Colorimetric recognition
KW - Fluorescent “turn-on”
KW - Nanomolar detection
KW - Photoinduced energy transfer
KW - Reversible Fe sensor
KW - Rhodamine-pyridine conjugate
UR - http://www.scopus.com/inward/record.url?scp=85089139562&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2020.118757
DO - 10.1016/j.saa.2020.118757
M3 - Article
C2 - 32791389
AN - SCOPUS:85089139562
SN - 1386-1425
VL - 242
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
M1 - 118757
ER -