TY - JOUR
T1 - Novel anthracene- and pyridine-containing Schiff base probe for selective "off-on" fluorescent determination of Cu2+ ions towards live cell application
AU - Simon, Turibius
AU - Shellaiah, Muthaiah
AU - Srinivasadesikan, Venkatesan
AU - Lin, Ching Chang
AU - Ko, Fu-Hsiang
AU - Sun, Kien-Wen
AU - Lin, Ming-Chang
N1 - Publisher Copyright:
© 2016 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.
PY - 2016
Y1 - 2016
N2 - The novel anthracene- and pyridine-containing Schiff base derivative 2-(2-(anthracen-9-ylmethylene)hydrazinyl)pyridine (AP) has been synthesized via one-pot reaction and its fluorescent "off-on" detection of Cu2+ ions, via PET-based mechanism, is reported. The 1:1 stoichiometry of AP⋯Cu2+ sensor complex was calculated from Job's plot based on PL titrations and supported by ESI (+Ve) mass spectral analysis. In addition, the binding sites of AP⋯Cu2+ sensor conjugate were supported by 1H NMR titration. The detection limit (LOD) and association constant (Ka) of AP⋯Cu2+ complex were obtained by standard deviation and linear fittings. Furthermore, quantum yield (Φf), SEM analysis, pH effect, FTIR interpretation and density functional theory (DFT) studies were conducted for the AP⋯Cu2+ sensing conjugate. More decisively, confocal fluorescence microscopy imaging from Raw264.7 cells indicated that AP could be used as an effective fluorescent probe for the analysis of Cu2+ ions in living cells.
AB - The novel anthracene- and pyridine-containing Schiff base derivative 2-(2-(anthracen-9-ylmethylene)hydrazinyl)pyridine (AP) has been synthesized via one-pot reaction and its fluorescent "off-on" detection of Cu2+ ions, via PET-based mechanism, is reported. The 1:1 stoichiometry of AP⋯Cu2+ sensor complex was calculated from Job's plot based on PL titrations and supported by ESI (+Ve) mass spectral analysis. In addition, the binding sites of AP⋯Cu2+ sensor conjugate were supported by 1H NMR titration. The detection limit (LOD) and association constant (Ka) of AP⋯Cu2+ complex were obtained by standard deviation and linear fittings. Furthermore, quantum yield (Φf), SEM analysis, pH effect, FTIR interpretation and density functional theory (DFT) studies were conducted for the AP⋯Cu2+ sensing conjugate. More decisively, confocal fluorescence microscopy imaging from Raw264.7 cells indicated that AP could be used as an effective fluorescent probe for the analysis of Cu2+ ions in living cells.
UR - http://www.scopus.com/inward/record.url?scp=84978064538&partnerID=8YFLogxK
U2 - 10.1039/c5nj03168k
DO - 10.1039/c5nj03168k
M3 - Article
AN - SCOPUS:84978064538
SN - 1144-0546
VL - 40
SP - 6101
EP - 6108
JO - New Journal of Chemistry
JF - New Journal of Chemistry
IS - 7
ER -