Nonlinear multi-modality spectro-microscopy: Multi-photon fluorescence, SHG and THG of biological specimen

Ping Chin Cheng; Chi Kuang Sun; Bai Ling Lin; Fu Jen Kao; Shi Wei Chu

doi:10.1117/12.424541

Nonlinear multi-modality spectro-microscopy: Multi-photon fluorescence, SHG and THG of biological specimen

Ping Chin Cheng, Chi Kuang Sun, Bai Ling Lin, Fu Jen Kao, Shi Wei Chu

Institute of Biophotonics

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry ar high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, bur also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG.

Original language	English
Pages (from-to)	98-103
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4262
DOIs	https://doi.org/10.1117/12.424541
State	Published - 2001

Keywords

Multi-photon fluorescence
Non-linear microscopy
SHG
THG

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title = "Nonlinear multi-modality spectro-microscopy: Multi-photon fluorescence, SHG and THG of biological specimen",

abstract = "The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry ar high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, bur also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG.",

keywords = "Multi-photon fluorescence, Non-linear microscopy, SHG, THG",

author = "Cheng, \{Ping Chin\} and Sun, \{Chi Kuang\} and Lin, \{Bai Ling\} and Kao, \{Fu Jen\} and Chu, \{Shi Wei\}",

year = "2001",

doi = "10.1117/12.424541",

language = "English",

volume = "4262",

pages = "98--103",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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TY - JOUR

T1 - Nonlinear multi-modality spectro-microscopy

T2 - Multi-photon fluorescence, SHG and THG of biological specimen

AU - Cheng, Ping Chin

AU - Sun, Chi Kuang

AU - Lin, Bai Ling

AU - Kao, Fu Jen

AU - Chu, Shi Wei

PY - 2001

Y1 - 2001

N2 - The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry ar high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, bur also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG.

AB - The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry ar high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, bur also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG.

KW - Multi-photon fluorescence

KW - Non-linear microscopy

KW - SHG

KW - THG

UR - https://www.scopus.com/pages/publications/0034942873

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DO - 10.1117/12.424541

M3 - Article

AN - SCOPUS:0034942873

SN - 0277-786X

VL - 4262

SP - 98

EP - 103

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

ER -

Nonlinear multi-modality spectro-microscopy: Multi-photon fluorescence, SHG and THG of biological specimen

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Keywords

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