Nonlinear multi-modality spectro-microscopy: Multi-photon fluorescence, SHG and THG of biological specimen

Ping Chin Cheng, Chi Kuang Sun, Bai Ling Lin, Fu Jen Kao, Shi Wei Chu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry ar high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, bur also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG.

Original languageEnglish
Pages (from-to)98-103
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4262
DOIs
StatePublished - 2001

Keywords

  • Multi-photon fluorescence
  • Non-linear microscopy
  • SHG
  • THG

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