Abstract
The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry ar high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, bur also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG.
Original language | English |
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Pages (from-to) | 98-103 |
Number of pages | 6 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 4262 |
DOIs | |
State | Published - 2001 |
Keywords
- Multi-photon fluorescence
- Non-linear microscopy
- SHG
- THG