Non-classical estrogen receptors action on human dermal fibroblasts

Kuan Hao Tsui; Peng Hui Wang; Chia Kai Chen; Yi Jen Chen; Shih Hwa Chiou; Yen Jen Sung; Hsin Yang Li

doi:10.1016/j.tjog.2011.10.013

Non-classical estrogen receptors action on human dermal fibroblasts

Kuan Hao Tsui, Peng Hui Wang, Chia Kai Chen, Yi Jen Chen, Shih Hwa Chiou, Yen Jen Sung, Hsin Yang Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Objective: To study the possible non-genomic effect of selective estrogen receptor modulators on human dermal fibroblasts (HDF). Materials and Methods: WS1 cells were used to test the effect of raloxifene. The mRNA expressions of estrogen receptor (ER) α and β and G protein-coupled ER 1(GRP30) were examined by reverse transcription polymerase chain reaction. Apoptosis was identified by TUNEL assay and FACS analysis. MAPK and PI3 K/Akt pathways were determined by immunoblotting analysis. Results: Neither ERα nor ERβ, but GPR30 was detected in WS1 cells. Raloxifene increased apoptosis, which was blocked by pertussis toxin, an inhibitor of G protein, or by LY294002. Phosphorylated p38 MAPK and Akt were also increased after raloxifene treatment. Conclusion: SERMs could induce apoptosis of HDF through G protein and PI3 K/Akt signaling, which may help understand the role of SERMs on the skin.

Original language	English
Pages (from-to)	474-478
Number of pages	5
Journal	Taiwanese Journal of Obstetrics and Gynecology
Volume	50
Issue number	4
DOIs	https://doi.org/10.1016/j.tjog.2011.10.013
State	Published - Dec 2011

Keywords

Apoptosis
Estrogen
Human dermal fibroblasts
Non-genomic
Selective estrogen receptor modulator

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10.1016/j.tjog.2011.10.013

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@article{30cb0e9e5769448fa5cdb12f197900bc,

title = "Non-classical estrogen receptors action on human dermal fibroblasts",

abstract = "Objective: To study the possible non-genomic effect of selective estrogen receptor modulators on human dermal fibroblasts (HDF). Materials and Methods: WS1 cells were used to test the effect of raloxifene. The mRNA expressions of estrogen receptor (ER) α and β and G protein-coupled ER 1(GRP30) were examined by reverse transcription polymerase chain reaction. Apoptosis was identified by TUNEL assay and FACS analysis. MAPK and PI3 K/Akt pathways were determined by immunoblotting analysis. Results: Neither ERα nor ERβ, but GPR30 was detected in WS1 cells. Raloxifene increased apoptosis, which was blocked by pertussis toxin, an inhibitor of G protein, or by LY294002. Phosphorylated p38 MAPK and Akt were also increased after raloxifene treatment. Conclusion: SERMs could induce apoptosis of HDF through G protein and PI3 K/Akt signaling, which may help understand the role of SERMs on the skin.",

keywords = "Apoptosis, Estrogen, Human dermal fibroblasts, Non-genomic, Selective estrogen receptor modulator",

author = "Tsui, {Kuan Hao} and Wang, {Peng Hui} and Chen, {Chia Kai} and Chen, {Yi Jen} and Chiou, {Shih Hwa} and Sung, {Yen Jen} and Li, {Hsin Yang}",

year = "2011",

month = dec,

doi = "10.1016/j.tjog.2011.10.013",

language = "English",

volume = "50",

pages = "474--478",

journal = "Taiwanese Journal of Obstetrics and Gynecology",

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}

TY - JOUR

T1 - Non-classical estrogen receptors action on human dermal fibroblasts

AU - Tsui, Kuan Hao

AU - Wang, Peng Hui

AU - Chen, Chia Kai

AU - Chen, Yi Jen

AU - Chiou, Shih Hwa

AU - Sung, Yen Jen

AU - Li, Hsin Yang

PY - 2011/12

Y1 - 2011/12

N2 - Objective: To study the possible non-genomic effect of selective estrogen receptor modulators on human dermal fibroblasts (HDF). Materials and Methods: WS1 cells were used to test the effect of raloxifene. The mRNA expressions of estrogen receptor (ER) α and β and G protein-coupled ER 1(GRP30) were examined by reverse transcription polymerase chain reaction. Apoptosis was identified by TUNEL assay and FACS analysis. MAPK and PI3 K/Akt pathways were determined by immunoblotting analysis. Results: Neither ERα nor ERβ, but GPR30 was detected in WS1 cells. Raloxifene increased apoptosis, which was blocked by pertussis toxin, an inhibitor of G protein, or by LY294002. Phosphorylated p38 MAPK and Akt were also increased after raloxifene treatment. Conclusion: SERMs could induce apoptosis of HDF through G protein and PI3 K/Akt signaling, which may help understand the role of SERMs on the skin.

AB - Objective: To study the possible non-genomic effect of selective estrogen receptor modulators on human dermal fibroblasts (HDF). Materials and Methods: WS1 cells were used to test the effect of raloxifene. The mRNA expressions of estrogen receptor (ER) α and β and G protein-coupled ER 1(GRP30) were examined by reverse transcription polymerase chain reaction. Apoptosis was identified by TUNEL assay and FACS analysis. MAPK and PI3 K/Akt pathways were determined by immunoblotting analysis. Results: Neither ERα nor ERβ, but GPR30 was detected in WS1 cells. Raloxifene increased apoptosis, which was blocked by pertussis toxin, an inhibitor of G protein, or by LY294002. Phosphorylated p38 MAPK and Akt were also increased after raloxifene treatment. Conclusion: SERMs could induce apoptosis of HDF through G protein and PI3 K/Akt signaling, which may help understand the role of SERMs on the skin.

KW - Apoptosis

KW - Estrogen

KW - Human dermal fibroblasts

KW - Non-genomic

KW - Selective estrogen receptor modulator

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U2 - 10.1016/j.tjog.2011.10.013

DO - 10.1016/j.tjog.2011.10.013

M3 - Article

C2 - 22212320

AN - SCOPUS:84855244163

SN - 1028-4559

VL - 50

SP - 474

EP - 478

JO - Taiwanese Journal of Obstetrics and Gynecology

JF - Taiwanese Journal of Obstetrics and Gynecology

IS - 4

ER -

Non-classical estrogen receptors action on human dermal fibroblasts

Abstract

Keywords

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