New structural proteins of Halobacterium salinarum gas vesicle revealed by comparative proteomics analysis

Lichieh Julie Chu, Mengchieh Claire Chen, Jocelyn Setter, Yihsuan Shannon Tsai, Hanyin Yang, Xuefeng Fang, Ying Sonia Ting, Scott A. Shaffer, Gregory K. Taylor, Priska D. Von Haller, David R. Goodlett, Wailap Victor Ng

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The Halobacterium salinarum gas vesicle (GV) is an extremely stable intracellular organelle with air trapped inside a proteinaceous membrane. Reported here is a comparative proteomics analysis of GV and GV depleted lysate (GVD) to reveal the membrane structural proteins. Ten proteins encoded by gvp-1 (gvpMLKJIHGFED-1 and gvpACNO-1) and five proteins encoded by gvp-2 (gvpMLKJIHGFED-2 and gvpACNO-2) gene clusters for the biogenesis of spindle- and cylindrical-, respectively, shaped GV were identified by LC-MS/MS. The peptides of GvpA1, I1, J1, A2, and J2 were exclusively identified in purified GV, GvpD1, H1, L1, and F2 only in GVD, and GvpC1, N1, O1, F1, H2, and O2 in both samples. The identification of GvpA1, C1, F1, J1, and A2 in GV is in agreement with their previously known structural function. In addition, the detection of GvpI1, N1, O1, H2, J2, and O2 in GV suggested they are new structural proteins. Among these, the structural role of GvpI1 and N1 in GV was further validated by immuno-detection of protein A-tagged GvpI1 and N1 fusion proteins in purified GV. Thus, LC-MS/MS could reveal at least a half dozen gas vesicle structural proteins in the predominant spindle-shaped GV that may be helpful for studying its biogenesis.

Original languageEnglish
Pages (from-to)1170-1178
Number of pages9
JournalJournal of Proteome Research
Volume10
Issue number3
DOIs
StatePublished - 4 Mar 2011

Keywords

  • Halobacterium salinarum
  • archaea
  • gas vesicle
  • proteomics
  • structural protein

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