TY - JOUR
T1 - Neuronal basis for pain-like and anxiety-like behaviors in the central nucleus of the amygdala
AU - Chen, Wei Hsin
AU - Lien, Cheng Chang
AU - Chen, Chien Chang
N1 - Publisher Copyright:
© 2022 Lippincott Williams and Wilkins. All rights reserved.
PY - 2022/3/1
Y1 - 2022/3/1
N2 - Chronic pain is often accompanied by anxiety and depression disorders. Amygdala nuclei play important roles in emotional responses, fear, depression, anxiety, and pain modulation. The exact mechanism of how amygdala neurons are involved in pain and anxiety is not completely understood. The central nucleus of the amygdala contains 2 major subpopulations of GABAergic neurons that express somatostatin (SOM+) or protein kinase Cδ (PKCδ+). In this study, we found about 70% of phosphorylated ERK-positive neurons colocalized with PKCδ+neurons in the formalin-induced pain model in mice. Optogenetic activation of PKCδ+neurons was sufficient to induce mechanical hyperalgesia without changing anxiety-like behavior in naïve mice. Conversely, chemogenetic inhibition of PKCδ+neurons significantly reduced the mechanical hyperalgesia in the pain model. By contrast, optogenetic inhibition of SOM+neurons induced mechanical hyperalgesia in naïve mice and increased phosphorylated ERK-positive neurons mainly in PKCδ+neurons. Optogenetic activation of SOM+neurons slightly reduced the mechanical hyperalgesia in the pain model but did not change the mechanical sensitivity in naïve mice. Instead, it induced anxiety-like behavior. Our results suggest that the PKCδ+and SOM+neurons in the central amygdala exert different functions in regulating pain-like and anxiety-like behaviors in mice.
AB - Chronic pain is often accompanied by anxiety and depression disorders. Amygdala nuclei play important roles in emotional responses, fear, depression, anxiety, and pain modulation. The exact mechanism of how amygdala neurons are involved in pain and anxiety is not completely understood. The central nucleus of the amygdala contains 2 major subpopulations of GABAergic neurons that express somatostatin (SOM+) or protein kinase Cδ (PKCδ+). In this study, we found about 70% of phosphorylated ERK-positive neurons colocalized with PKCδ+neurons in the formalin-induced pain model in mice. Optogenetic activation of PKCδ+neurons was sufficient to induce mechanical hyperalgesia without changing anxiety-like behavior in naïve mice. Conversely, chemogenetic inhibition of PKCδ+neurons significantly reduced the mechanical hyperalgesia in the pain model. By contrast, optogenetic inhibition of SOM+neurons induced mechanical hyperalgesia in naïve mice and increased phosphorylated ERK-positive neurons mainly in PKCδ+neurons. Optogenetic activation of SOM+neurons slightly reduced the mechanical hyperalgesia in the pain model but did not change the mechanical sensitivity in naïve mice. Instead, it induced anxiety-like behavior. Our results suggest that the PKCδ+and SOM+neurons in the central amygdala exert different functions in regulating pain-like and anxiety-like behaviors in mice.
KW - PKCδ+
KW - SOM+
KW - pERK, CeA, Anxiety, Pain, Optogenetics, Chemogenetics, Formalin model
UR - http://www.scopus.com/inward/record.url?scp=85124578138&partnerID=8YFLogxK
U2 - 10.1097/j.pain.0000000000002389
DO - 10.1097/j.pain.0000000000002389
M3 - Article
C2 - 34174041
AN - SCOPUS:85124578138
SN - 0304-3959
VL - 163
SP - E463-E475
JO - Pain
JF - Pain
IS - 3
ER -