Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

Mostafa Kamal Masud, Jongbeom Na, Tzu-En Lin, Victor Malgras, Anant Preet, Abu Ali Ibn Sina, Kathleen Wood, Mutasim Billah, Jeonghun Kim, Jungmok You, Kenya Kani, Andrew E. Whitten, Carlos Salomon, Nam-Trung Nguyen, Muhammad J. A. Shiddiky, Matt Trau, Md Shahriar A. Hossain, Yusuke Yamauchi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)(6)](3/4) expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe (CN)(6)](4/3) redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics.

Original languageEnglish
Article number112429
Number of pages9
JournalBiosensors and Bioelectronics
Volume168
DOIs
StatePublished - 15 Nov 2020

Keywords

  • Nanoarchitectonics
  • Mesoporous gold electrode
  • Biosensor
  • microRNA
  • Electrocatalytic activity
  • Electrochemical detection
  • ELECTROCATALYTIC DETECTION
  • ELECTROCHEMICAL DETECTION
  • SIGNAL AMPLIFICATION
  • CANCER
  • NANOCUBES
  • BIOMARKER
  • GRAPHENE
  • PCR

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