Nanometer-sized diamond particle as a probe for biolabeling

Jui-I Chao, Elena Perevedentseva, Pei Hua Chung, Kuang Kai Liu, Chih Yuan Cheng, Chia-Ching Chang, Chia Liang Cheng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

252 Scopus citations


A novel method is proposed using nanometer-sized diamond particles as detection probes for biolabeling. The advantages of nanodiamond's unique properties were demonstrated in its biocompatibility, nontoxicity, easily detected Raman signal, and intrinsic fluorescence from its natural defects without complicated pretreatments. Carboxylated nanodiamond's (cND's) penetration ability, noncytotoxicity, and visualization of cND-cell interactions are demonstrated on A549 human lung epithelial cells. Protein-targeted cell interaction visualization was demonstrated with cND-lysozyme complex interaction with bacteria Escherichia coli. It is shown that the developed biomolecule-cND complex preserves the original functions of the test protein. The easily detected natural fluorescent and Raman intrinsic signals, penetration ability, and low cytotoxicity of cNDs render them promising agents in multiple medical applications.

Original languageEnglish
Pages (from-to)2199-2208
Number of pages10
JournalBiophysical Journal
Issue number6
StatePublished - Sep 2007


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