Multispectral fluorescence diffuse optical tomography

Pei An Lo, Jaedu Cho, Farouk Nouizi, Huihua Kenny Chiang, Gultekin Gulsen*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Fluorescence diffuse optical tomography (FDOT) has been widely used for in vivo small animal studies and the illposed problem in reconstruction can be eased by utilizing structural a priori obtained from an anatomic imaging modality. In this study, a multispectral fluorescence tomography (FT) is used, which has shown the ability to detect subtle shifts in the ICG absorption spectrum in our previous study. The imaging system is in trans-illumination mode with a swept-wavelength laser and a CCD on a rotation gantry and the structural image from the X-ray computed tomography is used to guide and constrain the FT reconstruction algorithm. In this work, a phantom with two inclusions filled with different fluorophores is utilized to evaluate whether the spectral information obtained using sweptwavelength laser can distinguish these two inclusions. The images are captured from 8 different views with three different wavelengths.

Original languageEnglish
Title of host publicationDesign and Quality for Biomedical Technologies X
EditorsRamesh Raghavachari, Rongguang Liang
ISBN (Electronic)9781510605534
StatePublished - 2017
EventDesign and Quality for Biomedical Technologies X - San Francisco, United States
Duration: 28 Jan 201729 Jan 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceDesign and Quality for Biomedical Technologies X
Country/TerritoryUnited States
CitySan Francisco


  • Diffuse Optical Tomography
  • Fluorescence Tomography
  • Frequency Domain
  • Multi-wavelength
  • NIR Swept Laser
  • Optical Imaging


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