Multiple approaches to characterize the microbial community in a thermophilic anaerobic digester running on swine manure: A case study

Nguyen Ngoc Tuan, Yi Chia Chang, Chang Ping Yu, Shir Ly Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

In this study, the first survey of microbial community in thermophilic anaerobic digester using swine manure as sole feedstock was performed by multiple approaches including denaturing gradient gel electrophoresis (DGGE), clone library and pyrosequencing techniques. The integrated analysis of 21 DGGE bands, 126 clones and 8506 pyrosequencing read sequences revealed that Clostridia from the phylum Firmicutes account for the most dominant Bacteria. In addition, our analysis also identified additional taxa that were missed by the previous researches, including members of the bacterial phyla Synergistetes, Planctomycetes, Armatimonadetes, Chloroflexi and Nitrospira which might also play a role in thermophilic anaerobic digester. Most archaeal 16S rRNA sequences could be assigned to the order Methanobacteriales instead of Methanomicrobiales comparing to previous studies. In addition, this study reported that the member of Methanothermobacter genus was firstly found in thermophilic anaerobic digester.

Original languageEnglish
Pages (from-to)717-724
Number of pages8
JournalMicrobiological Research
Volume169
Issue number9-10
DOIs
StatePublished - Sep 2014

Keywords

  • 16S rRNA
  • Anaerobic digestion
  • Metagenome
  • Pyrosequencing

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