Multiphoton imaging and quantification of tissue glycation

Ara A. Ghazaryan; Jo Ya Tseng; Wen Lo; Yang Fang Chen; Vladimir Hovhannisyan; Shean-Jen Chen; Hsin Yuan Tan; Chen Yuan Dong

doi:10.1117/12.874536

Multiphoton imaging and quantification of tissue glycation

Ara A. Ghazaryan, Jo Ya Tseng, Wen Lo, Yang Fang Chen, Vladimir Hovhannisyan, Shean-Jen Chen, Hsin Yuan Tan^*, Chen Yuan Dong

^*Corresponding author for this work

Institute of Imaging and Biomedical Photonics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Scopus citations

Abstract

In this work, we applied multi-photon microscopy to image and characterize the extent of tissue glycation. Specifically, features of multi-photon autofluorescence (MPAF) and second harmonic generation (SHG) images were presented. Our study shows that multiphoton imaging is capable of providing qualitative and quantitative information of the extent of tissue glycation in ribose treated samples including bovine sclera and vessel and that this approach may be useful for clinical evaluation of advanced glycation endproducts (AGEs) formation.

Original language	English
Title of host publication	Optical Biopsy IX
DOIs	https://doi.org/10.1117/12.874536
State	Published - 21 Apr 2011
Event	Optical Biopsy IX - San Francisco, CA, United States Duration: 24 Jan 2011 → 26 Jan 2011

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7895
ISSN (Print)	1605-7422

Conference

Conference	Optical Biopsy IX
Country/Territory	United States
City	San Francisco, CA
Period	24/01/11 → 26/01/11

Keywords

advanced glycation product
glycation
multiphoton microscopy
spectral imaging

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10.1117/12.874536

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title = "Multiphoton imaging and quantification of tissue glycation",

abstract = "In this work, we applied multi-photon microscopy to image and characterize the extent of tissue glycation. Specifically, features of multi-photon autofluorescence (MPAF) and second harmonic generation (SHG) images were presented. Our study shows that multiphoton imaging is capable of providing qualitative and quantitative information of the extent of tissue glycation in ribose treated samples including bovine sclera and vessel and that this approach may be useful for clinical evaluation of advanced glycation endproducts (AGEs) formation.",

keywords = "advanced glycation product, glycation, multiphoton microscopy, spectral imaging",

author = "Ghazaryan, {Ara A.} and Tseng, {Jo Ya} and Wen Lo and Chen, {Yang Fang} and Vladimir Hovhannisyan and Shean-Jen Chen and Tan, {Hsin Yuan} and Dong, {Chen Yuan}",

year = "2011",

month = apr,

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doi = "10.1117/12.874536",

language = "English",

isbn = "9780819484321",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Optical Biopsy IX",

note = "Optical Biopsy IX ; Conference date: 24-01-2011 Through 26-01-2011",

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TY - GEN

T1 - Multiphoton imaging and quantification of tissue glycation

AU - Ghazaryan, Ara A.

AU - Tseng, Jo Ya

AU - Lo, Wen

AU - Chen, Yang Fang

AU - Hovhannisyan, Vladimir

AU - Chen, Shean-Jen

AU - Tan, Hsin Yuan

AU - Dong, Chen Yuan

PY - 2011/4/21

Y1 - 2011/4/21

N2 - In this work, we applied multi-photon microscopy to image and characterize the extent of tissue glycation. Specifically, features of multi-photon autofluorescence (MPAF) and second harmonic generation (SHG) images were presented. Our study shows that multiphoton imaging is capable of providing qualitative and quantitative information of the extent of tissue glycation in ribose treated samples including bovine sclera and vessel and that this approach may be useful for clinical evaluation of advanced glycation endproducts (AGEs) formation.

AB - In this work, we applied multi-photon microscopy to image and characterize the extent of tissue glycation. Specifically, features of multi-photon autofluorescence (MPAF) and second harmonic generation (SHG) images were presented. Our study shows that multiphoton imaging is capable of providing qualitative and quantitative information of the extent of tissue glycation in ribose treated samples including bovine sclera and vessel and that this approach may be useful for clinical evaluation of advanced glycation endproducts (AGEs) formation.

KW - advanced glycation product

KW - glycation

KW - multiphoton microscopy

KW - spectral imaging

UR - http://www.scopus.com/inward/record.url?scp=79954533747&partnerID=8YFLogxK

U2 - 10.1117/12.874536

DO - 10.1117/12.874536

M3 - Conference contribution

AN - SCOPUS:79954533747

SN - 9780819484321

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Biopsy IX

T2 - Optical Biopsy IX

Y2 - 24 January 2011 through 26 January 2011

ER -

Multiphoton imaging and quantification of tissue glycation

Abstract

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Keywords

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