Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine

Shu Jen Chiang; Po Hang Tseng; Hsu Cheng Huang; Shih Chi Chen; Shean-Jen Chen; Mei Chun Lin; Pei Jen Lou; Chen Yuan Dong

doi:10.1117/12.2545617

Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine

Shu Jen Chiang^*, Po Hang Tseng, Hsu Cheng Huang, Shih Chi Chen, Shean-Jen Chen, Mei Chun Lin, Pei Jen Lou, Chen Yuan Dong

^*Corresponding author for this work

Institute of Imaging and Biomedical Photonics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Traditionally, testing of therapeutic agents uses two-dimensional cell cultures which does not recapitulate the complex, three-dimensional architecture of tissues. In the case of cancer drug development, immunotherapy (IT) emerged as an effective treatment strategy for the patients who respond to therapy. However, the overall patient response rate for immunotherapy is around 25%. Therefore, there is a need to develop more effective drug testing platform for cancer patients. In this work, we develop three-dimensional tissue cultures of human oral cancer slices. We observed the growth dynamics of the tissue sections for up to 28 days. Their viability over extended periods suggest that the tissue sections may be used for personalized drug testing.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XX
Editors	Ammasi Periasamy, Peter T. C. So, Karsten Konig, Karsten Konig
Publisher	SPIE
Number of pages	5
ISBN (Electronic)	9781510632516
DOIs	https://doi.org/10.1117/12.2545617
State	Published - 21 Feb 2020
Event	Multiphoton Microscopy in the Biomedical Sciences XX 2020 - San Francisco, United States Duration: 2 Feb 2020 → 4 Feb 2020

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11244
ISSN (Print)	1605-7422

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XX 2020
Country/Territory	United States
City	San Francisco
Period	2/02/20 → 4/02/20

Keywords

Multiphoton Imaging
Oral Cancer
Precision Medicine
Tumor Sections

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.2545617

Cite this

Chiang, S. J., Tseng, P. H., Huang, H. C., Chen, S. C., Chen, S.-J., Lin, M. C., Lou, P. J., & Dong, C. Y. (2020). Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine. In A. Periasamy, P. T. C. So, K. Konig, & K. Konig (Eds.), Multiphoton Microscopy in the Biomedical Sciences XX Article 1124427 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11244). SPIE. https://doi.org/10.1117/12.2545617

Chiang, Shu Jen ; Tseng, Po Hang ; Huang, Hsu Cheng et al. / Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine. Multiphoton Microscopy in the Biomedical Sciences XX. editor / Ammasi Periasamy ; Peter T. C. So ; Karsten Konig ; Karsten Konig. SPIE, 2020. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine",

abstract = "Traditionally, testing of therapeutic agents uses two-dimensional cell cultures which does not recapitulate the complex, three-dimensional architecture of tissues. In the case of cancer drug development, immunotherapy (IT) emerged as an effective treatment strategy for the patients who respond to therapy. However, the overall patient response rate for immunotherapy is around 25%. Therefore, there is a need to develop more effective drug testing platform for cancer patients. In this work, we develop three-dimensional tissue cultures of human oral cancer slices. We observed the growth dynamics of the tissue sections for up to 28 days. Their viability over extended periods suggest that the tissue sections may be used for personalized drug testing.",

keywords = "Multiphoton Imaging, Oral Cancer, Precision Medicine, Tumor Sections",

author = "Chiang, {Shu Jen} and Tseng, {Po Hang} and Huang, {Hsu Cheng} and Chen, {Shih Chi} and Shean-Jen Chen and Lin, {Mei Chun} and Lou, {Pei Jen} and Dong, {Chen Yuan}",

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booktitle = "Multiphoton Microscopy in the Biomedical Sciences XX",

address = "United States",

note = "Multiphoton Microscopy in the Biomedical Sciences XX 2020 ; Conference date: 02-02-2020 Through 04-02-2020",

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Chiang, SJ, Tseng, PH, Huang, HC, Chen, SC, Chen, S-J, Lin, MC, Lou, PJ & Dong, CY 2020, Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine. in A Periasamy, PTC So, K Konig & K Konig (eds), Multiphoton Microscopy in the Biomedical Sciences XX., 1124427, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11244, SPIE, Multiphoton Microscopy in the Biomedical Sciences XX 2020, San Francisco, United States, 2/02/20. https://doi.org/10.1117/12.2545617

Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine. / Chiang, Shu Jen; Tseng, Po Hang; Huang, Hsu Cheng et al.
Multiphoton Microscopy in the Biomedical Sciences XX. ed. / Ammasi Periasamy; Peter T. C. So; Karsten Konig; Karsten Konig. SPIE, 2020. 1124427 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11244).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine

AU - Chiang, Shu Jen

AU - Tseng, Po Hang

AU - Huang, Hsu Cheng

AU - Chen, Shih Chi

AU - Chen, Shean-Jen

AU - Lin, Mei Chun

AU - Lou, Pei Jen

AU - Dong, Chen Yuan

PY - 2020/2/21

Y1 - 2020/2/21

N2 - Traditionally, testing of therapeutic agents uses two-dimensional cell cultures which does not recapitulate the complex, three-dimensional architecture of tissues. In the case of cancer drug development, immunotherapy (IT) emerged as an effective treatment strategy for the patients who respond to therapy. However, the overall patient response rate for immunotherapy is around 25%. Therefore, there is a need to develop more effective drug testing platform for cancer patients. In this work, we develop three-dimensional tissue cultures of human oral cancer slices. We observed the growth dynamics of the tissue sections for up to 28 days. Their viability over extended periods suggest that the tissue sections may be used for personalized drug testing.

AB - Traditionally, testing of therapeutic agents uses two-dimensional cell cultures which does not recapitulate the complex, three-dimensional architecture of tissues. In the case of cancer drug development, immunotherapy (IT) emerged as an effective treatment strategy for the patients who respond to therapy. However, the overall patient response rate for immunotherapy is around 25%. Therefore, there is a need to develop more effective drug testing platform for cancer patients. In this work, we develop three-dimensional tissue cultures of human oral cancer slices. We observed the growth dynamics of the tissue sections for up to 28 days. Their viability over extended periods suggest that the tissue sections may be used for personalized drug testing.

KW - Multiphoton Imaging

KW - Oral Cancer

KW - Precision Medicine

KW - Tumor Sections

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M3 - Conference contribution

AN - SCOPUS:85082644957

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Multiphoton Microscopy in the Biomedical Sciences XX

A2 - Periasamy, Ammasi

A2 - So, Peter T. C.

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PB - SPIE

T2 - Multiphoton Microscopy in the Biomedical Sciences XX 2020

Y2 - 2 February 2020 through 4 February 2020

ER -

Chiang SJ, Tseng PH, Huang HC, Chen SC, Chen SJ, Lin MC et al. Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine. In Periasamy A, So PTC, Konig K, Konig K, editors, Multiphoton Microscopy in the Biomedical Sciences XX. SPIE. 2020. 1124427. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2545617

Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine

Abstract

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Conference

Keywords

UN SDGs

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