Multi-scale mapping for collagen-regulated mineralization in bone remodeling of additive manufacturing porous implants

Pei I. Tsai; Tu Ngoc Lam; Meng Huang Wu; Kuan Ying Tseng; Yuan Wei Chang; Jui Sheng Sun; Yen Yao Li; Ming Hsueh Lee; San-Yuan Chen; Chung Kai Chang; Chun Jen Su; Chia Hsien Lin; Ching Yu Chiang; Ching Shun Ku; Nien-Ti Tsou; Shao Ju Shih; Chun Chieh Wang; E-Wen Huang

doi:10.1016/j.matchemphys.2019.03.047

Multi-scale mapping for collagen-regulated mineralization in bone remodeling of additive manufacturing porous implants

Pei I. Tsai, Tu Ngoc Lam, Meng Huang Wu, Kuan Ying Tseng, Yuan Wei Chang, Jui Sheng Sun, Yen Yao Li, Ming Hsueh Lee, San-Yuan Chen, Chung Kai Chang, Chun Jen Su, Chia Hsien Lin, Ching Yu Chiang, Ching Shun Ku, Nien-Ti Tsou, Shao Ju Shih, Chun Chieh Wang, E-Wen Huang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Long term success of metallic fusion cages depends on mechanobiological processes through the bone incorporation and rich osseointegration. An optimal configuration of porous titanium-aluminum-vanadium (Ti–6Al–4V) implant fabricated via the additive manufacturing was evaluated in complimentary structure examinations to investigate the growth of autologous osseous at multi-length scales. X-ray microcomputed tomography (micro-CT) and transmission X-ray microscopy (TXM) using newly-built analysis method indicate the porous Ti–6Al–4V is much better for bone ingrowth compared to commercially non-porous titanium (Ti) and porous tantalum (Ta) implants at the ultramicrostructural level. The evolution of bone formation and remodeling acquired by nano X-ray Laue diffraction mapping exhibits the isotropic orientation and low crystallinity of all newly formed bone whereas mature bone in Ti–6Al–4V discloses the preferential alignment and higher crystallinity volumes of constituent hydroxyapatite (HA) crystallites. The high degree in mineral crystallinity of the fully mature bone suggests additive manufactured Ti–6Al–4V pores enhance the collagen-regulated mineralization.

Original language	English
Pages (from-to)	83-92
Number of pages	10
Journal	Materials Chemistry and Physics
Volume	230
DOIs	https://doi.org/10.1016/j.matchemphys.2019.03.047
State	Published - 15 May 2019

Keywords

Implant
Additive manufacturing
X-ray tomography
Small X-ray angle scattering
Nano X-ray Laue diffraction mapping
TANTALUM IMPLANTS
TITANIUM
REGENERATION
TISSUE
NUCLEATION
FUSION

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Tsai, P. I., Lam, T. N., Wu, M. H., Tseng, K. Y., Chang, Y. W., Sun, J. S., Li, Y. Y., Lee, M. H., Chen, S-Y., Chang, C. K., Su, C. J., Lin, C. H., Chiang, C. Y., Ku, C. S., Tsou, N-T., Shih, S. J., Wang, C. C., & Huang, E-W. (2019). Multi-scale mapping for collagen-regulated mineralization in bone remodeling of additive manufacturing porous implants. Materials Chemistry and Physics, 230, 83-92. https://doi.org/10.1016/j.matchemphys.2019.03.047

@article{9d4517b448ed4df6863aea2aa6f90628,

title = "Multi-scale mapping for collagen-regulated mineralization in bone remodeling of additive manufacturing porous implants",

abstract = "Long term success of metallic fusion cages depends on mechanobiological processes through the bone incorporation and rich osseointegration. An optimal configuration of porous titanium-aluminum-vanadium (Ti–6Al–4V) implant fabricated via the additive manufacturing was evaluated in complimentary structure examinations to investigate the growth of autologous osseous at multi-length scales. X-ray microcomputed tomography (micro-CT) and transmission X-ray microscopy (TXM) using newly-built analysis method indicate the porous Ti–6Al–4V is much better for bone ingrowth compared to commercially non-porous titanium (Ti) and porous tantalum (Ta) implants at the ultramicrostructural level. The evolution of bone formation and remodeling acquired by nano X-ray Laue diffraction mapping exhibits the isotropic orientation and low crystallinity of all newly formed bone whereas mature bone in Ti–6Al–4V discloses the preferential alignment and higher crystallinity volumes of constituent hydroxyapatite (HA) crystallites. The high degree in mineral crystallinity of the fully mature bone suggests additive manufactured Ti–6Al–4V pores enhance the collagen-regulated mineralization.",

keywords = "Implant, Additive manufacturing, X-ray tomography, Small X-ray angle scattering, Nano X-ray Laue diffraction mapping, TANTALUM IMPLANTS, TITANIUM, REGENERATION, TISSUE, NUCLEATION, FUSION",

author = "Tsai, {Pei I.} and Lam, {Tu Ngoc} and Wu, {Meng Huang} and Tseng, {Kuan Ying} and Chang, {Yuan Wei} and Sun, {Jui Sheng} and Li, {Yen Yao} and Lee, {Ming Hsueh} and San-Yuan Chen and Chang, {Chung Kai} and Su, {Chun Jen} and Lin, {Chia Hsien} and Chiang, {Ching Yu} and Ku, {Ching Shun} and Nien-Ti Tsou and Shih, {Shao Ju} and Wang, {Chun Chieh} and E-Wen Huang",

year = "2019",

month = may,

day = "15",

doi = "10.1016/j.matchemphys.2019.03.047",

language = "English",

volume = "230",

pages = "83--92",

journal = "Materials Chemistry and Physics",

issn = "0254-0584",

publisher = "Elsevier BV",

}

Tsai, PI, Lam, TN, Wu, MH, Tseng, KY, Chang, YW, Sun, JS, Li, YY, Lee, MH, Chen, S-Y, Chang, CK, Su, CJ, Lin, CH, Chiang, CY, Ku, CS, Tsou, N-T, Shih, SJ, Wang, CC & Huang, E-W 2019, 'Multi-scale mapping for collagen-regulated mineralization in bone remodeling of additive manufacturing porous implants', Materials Chemistry and Physics, vol. 230, pp. 83-92. https://doi.org/10.1016/j.matchemphys.2019.03.047

TY - JOUR

T1 - Multi-scale mapping for collagen-regulated mineralization in bone remodeling of additive manufacturing porous implants

AU - Tsai, Pei I.

AU - Lam, Tu Ngoc

AU - Wu, Meng Huang

AU - Tseng, Kuan Ying

AU - Chang, Yuan Wei

AU - Sun, Jui Sheng

AU - Li, Yen Yao

AU - Lee, Ming Hsueh

AU - Chen, San-Yuan

AU - Chang, Chung Kai

AU - Su, Chun Jen

AU - Lin, Chia Hsien

AU - Chiang, Ching Yu

AU - Ku, Ching Shun

AU - Tsou, Nien-Ti

AU - Shih, Shao Ju

AU - Wang, Chun Chieh

AU - Huang, E-Wen

PY - 2019/5/15

Y1 - 2019/5/15

N2 - Long term success of metallic fusion cages depends on mechanobiological processes through the bone incorporation and rich osseointegration. An optimal configuration of porous titanium-aluminum-vanadium (Ti–6Al–4V) implant fabricated via the additive manufacturing was evaluated in complimentary structure examinations to investigate the growth of autologous osseous at multi-length scales. X-ray microcomputed tomography (micro-CT) and transmission X-ray microscopy (TXM) using newly-built analysis method indicate the porous Ti–6Al–4V is much better for bone ingrowth compared to commercially non-porous titanium (Ti) and porous tantalum (Ta) implants at the ultramicrostructural level. The evolution of bone formation and remodeling acquired by nano X-ray Laue diffraction mapping exhibits the isotropic orientation and low crystallinity of all newly formed bone whereas mature bone in Ti–6Al–4V discloses the preferential alignment and higher crystallinity volumes of constituent hydroxyapatite (HA) crystallites. The high degree in mineral crystallinity of the fully mature bone suggests additive manufactured Ti–6Al–4V pores enhance the collagen-regulated mineralization.

AB - Long term success of metallic fusion cages depends on mechanobiological processes through the bone incorporation and rich osseointegration. An optimal configuration of porous titanium-aluminum-vanadium (Ti–6Al–4V) implant fabricated via the additive manufacturing was evaluated in complimentary structure examinations to investigate the growth of autologous osseous at multi-length scales. X-ray microcomputed tomography (micro-CT) and transmission X-ray microscopy (TXM) using newly-built analysis method indicate the porous Ti–6Al–4V is much better for bone ingrowth compared to commercially non-porous titanium (Ti) and porous tantalum (Ta) implants at the ultramicrostructural level. The evolution of bone formation and remodeling acquired by nano X-ray Laue diffraction mapping exhibits the isotropic orientation and low crystallinity of all newly formed bone whereas mature bone in Ti–6Al–4V discloses the preferential alignment and higher crystallinity volumes of constituent hydroxyapatite (HA) crystallites. The high degree in mineral crystallinity of the fully mature bone suggests additive manufactured Ti–6Al–4V pores enhance the collagen-regulated mineralization.

KW - Implant

KW - Additive manufacturing

KW - X-ray tomography

KW - Small X-ray angle scattering

KW - Nano X-ray Laue diffraction mapping

KW - TANTALUM IMPLANTS

KW - TITANIUM

KW - REGENERATION

KW - TISSUE

KW - NUCLEATION

KW - FUSION

UR - http://www.scopus.com/inward/record.url?scp=85063609217&partnerID=8YFLogxK

U2 - 10.1016/j.matchemphys.2019.03.047

DO - 10.1016/j.matchemphys.2019.03.047

M3 - Article

AN - SCOPUS:85063609217

SN - 0254-0584

VL - 230

SP - 83

EP - 92

JO - Materials Chemistry and Physics

JF - Materials Chemistry and Physics

ER -

Multi-scale mapping for collagen-regulated mineralization in bone remodeling of additive manufacturing porous implants

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Keywords

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