Abstract
The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.
Original language | English |
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Pages (from-to) | 100-104 |
Number of pages | 5 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 4082 |
DOIs | |
State | Published - 2000 |
Event | Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan Duration: 26 Jul 2000 → 28 Jul 2000 |