Multi-photon micro-spectroscopy of biological specimens

Bai Ling Lin*, Fu Jen Kao, Ping chin Cheng, Chi Kuang Sun, Rang Wu Chen, Yi min Wang, Jian cheng Chen, Yung Shun Wang, Tzu Ming Liu, Mao Kuo Huang

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations


The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2000
EventOptical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan
Duration: 26 Jul 200028 Jul 2000


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