The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.
|Number of pages
|Proceedings of SPIE - The International Society for Optical Engineering
|Published - 2000
|Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan
Duration: 26 Jul 2000 → 28 Jul 2000