Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set-up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.
|Number of pages
|Proceedings of SPIE - The International Society for Optical Engineering
|Published - Jul 2000
|Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan
Duration: 26 Jul 2000 → 28 Jul 2000