Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules

Ping chin Cheng*, Bai Ling Lin, Fu Jen Kao, Chi Kuang Sun, Yung Sheng Wang, Tzu Ming Liu, Yi min Wang, Jian cheng Chen, Mao Kuo Huang, I. Johnson

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

8 Scopus citations


Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set-up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

Original languageEnglish
Pages (from-to)87-91
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Jul 2000
EventOptical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan
Duration: 26 Jul 200028 Jul 2000


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