Multi-photon fluorescence micro-spectroscopy

Fu Jen Kao*, Bai Ling Lin, Ping chin Cheng

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations


The intrinsic confined photo-interacting volume in multi-photon fluorescence microscopy provides the possibility of obtaining fluorescence spectrum from specific cellular structure in a tissue. In this article, we demonstrated that it is feasible to obtain useful two-photon pumped fluorescence spectrum from cell wall and single chloroplast. The difference in fluorescence spectra obtained with single- and two-photon excitation indicates that a significant shift in fluorescence maximum may occur due to the non-linear nature of excitation. Therefore, in order to properly interpret two-photon fluorescence micrographs, it is important to characterize the fluorescence spectrum of the specimen and the commonly used fluorescence probes. The fluorescence spectra will in turn be useful in the selection of filter sets in multi-photon fluorescence microscopy.

Original languageEnglish
Pages (from-to)2-8
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - May 2000
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII - San Jose, CA, USA
Duration: 23 Jan 200024 Jan 2000


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