Mueller matrix signature in advanced fluorescence microscopy imaging

Nirmal Mazumder, Jianjun Qiu, Fu Jen Kao, Alberto Diaspro

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes-Mueller polarization microscopy. Thus, Lu-Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.

Original languageEnglish
Article number025301
JournalJournal of Optics (United Kingdom)
Volume19
Issue number2
DOIs
StatePublished - Feb 2017

Keywords

  • fluorescence microscopy
  • imaging systems
  • microscopy
  • polarimetric imaging

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