Abstract
We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes-Mueller polarization microscopy. Thus, Lu-Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.
Original language | English |
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Article number | 025301 |
Journal | Journal of Optics (United Kingdom) |
Volume | 19 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2017 |
Keywords
- fluorescence microscopy
- imaging systems
- microscopy
- polarimetric imaging