Monitoring cellular metabolism of 3T3 upon wild type E.coli infection by mapping NADH with FLIM

Tatyana Buryakina, Pin Tzu Su, Vladimir Gukassyan, Wan Jr Syu, Fu Jen Kao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Fluorescence lifetime imaging microscopy (FLIM) has gained popularity as a sensitive technique to monitor the functional/conformational states of reduced nicotinamide adenine dinucleotide (NADH), one of the main compounds of oxidative phosphorylation. In this letter, we apply the technique to characterize the metabolic changes in mouse embryonic fibroblast 3T3 cells upon bacterial infection. A gradual shortening of the decaying time constants in both the short and the long lifetime components of NADH's autofluorescence is detected. The ratio of the short and the long lifetime components' relative contributions, however, shows a rapid increase, indicating the rise of cellular metabolic activity over the course of infection.

Original languageEnglish
Pages (from-to)931-933
Number of pages3
JournalChinese Optics Letters
Volume8
Issue number10
DOIs
StatePublished - Oct 2010

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