Modulation of microsomal cytochrome P450 by Scutellariae Radix and Gentianae Scabrae Radix in rat liver

Jaw Jou Kang; Yi Chien Chen; Wei Chung Kuo; Terri Chen; Yu Wen Cheng; Min Liang Kuo; Tzuu Huei Ueng

doi:10.1142/S0192415X96000049

Modulation of microsomal cytochrome P450 by Scutellariae Radix and Gentianae Scabrae Radix in rat liver

Jaw Jou Kang^*, Yi Chien Chen, Wei Chung Kuo, Terri Chen, Yu Wen Cheng, Min Liang Kuo, Tzuu Huei Ueng

^*Corresponding author for this work

Institute of Food Safety and Health Risk Assessment

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Abstract

The present study has determined the effects of Scutellariae Radix(Huangqin) and Gentianae scabrae Radix (Longdan) on liver microsomal cytochrome P450 (P450)-dependent mono-oxygenases using rats pretreated with crude extracts of medicinal herbs. Scutellariae Radix resulted in a 53% decrease of pentoxyresorufin O-dealkylase activity in liver microsomes. In contrast, Gentianae scabrae Radix caused a 50% increase of benzo(a)pyrene hydroxylase activity. Immunoblotting analysis of liver microsomes revealed that Scutellariae Radix induced and suppressed the levels of P450 1A and 2B proteins, respectively. Scutellariae and Gentianae scabrae Radixes had no effects on microsomal aniline hydroxylase activity and P450 2E1 protein.

Original language	English
Pages (from-to)	19-29
Number of pages	11
Journal	American Journal of Chinese Medicine
Volume	24
Issue number	1
DOIs	https://doi.org/10.1142/S0192415X96000049
State	Published - 1996

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title = "Modulation of microsomal cytochrome P450 by Scutellariae Radix and Gentianae Scabrae Radix in rat liver",

abstract = "The present study has determined the effects of Scutellariae Radix(Huangqin) and Gentianae scabrae Radix (Longdan) on liver microsomal cytochrome P450 (P450)-dependent mono-oxygenases using rats pretreated with crude extracts of medicinal herbs. Scutellariae Radix resulted in a 53% decrease of pentoxyresorufin O-dealkylase activity in liver microsomes. In contrast, Gentianae scabrae Radix caused a 50% increase of benzo(a)pyrene hydroxylase activity. Immunoblotting analysis of liver microsomes revealed that Scutellariae Radix induced and suppressed the levels of P450 1A and 2B proteins, respectively. Scutellariae and Gentianae scabrae Radixes had no effects on microsomal aniline hydroxylase activity and P450 2E1 protein.",

author = "Kang, {Jaw Jou} and Chen, {Yi Chien} and Kuo, {Wei Chung} and Terri Chen and Cheng, {Yu Wen} and Kuo, {Min Liang} and Ueng, {Tzuu Huei}",

year = "1996",

doi = "10.1142/S0192415X96000049",

language = "English",

volume = "24",

pages = "19--29",

journal = "American Journal of Chinese Medicine",

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TY - JOUR

T1 - Modulation of microsomal cytochrome P450 by Scutellariae Radix and Gentianae Scabrae Radix in rat liver

AU - Kang, Jaw Jou

AU - Chen, Yi Chien

AU - Kuo, Wei Chung

AU - Chen, Terri

AU - Cheng, Yu Wen

AU - Kuo, Min Liang

AU - Ueng, Tzuu Huei

PY - 1996

Y1 - 1996

N2 - The present study has determined the effects of Scutellariae Radix(Huangqin) and Gentianae scabrae Radix (Longdan) on liver microsomal cytochrome P450 (P450)-dependent mono-oxygenases using rats pretreated with crude extracts of medicinal herbs. Scutellariae Radix resulted in a 53% decrease of pentoxyresorufin O-dealkylase activity in liver microsomes. In contrast, Gentianae scabrae Radix caused a 50% increase of benzo(a)pyrene hydroxylase activity. Immunoblotting analysis of liver microsomes revealed that Scutellariae Radix induced and suppressed the levels of P450 1A and 2B proteins, respectively. Scutellariae and Gentianae scabrae Radixes had no effects on microsomal aniline hydroxylase activity and P450 2E1 protein.

AB - The present study has determined the effects of Scutellariae Radix(Huangqin) and Gentianae scabrae Radix (Longdan) on liver microsomal cytochrome P450 (P450)-dependent mono-oxygenases using rats pretreated with crude extracts of medicinal herbs. Scutellariae Radix resulted in a 53% decrease of pentoxyresorufin O-dealkylase activity in liver microsomes. In contrast, Gentianae scabrae Radix caused a 50% increase of benzo(a)pyrene hydroxylase activity. Immunoblotting analysis of liver microsomes revealed that Scutellariae Radix induced and suppressed the levels of P450 1A and 2B proteins, respectively. Scutellariae and Gentianae scabrae Radixes had no effects on microsomal aniline hydroxylase activity and P450 2E1 protein.

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U2 - 10.1142/S0192415X96000049

DO - 10.1142/S0192415X96000049

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SN - 0192-415X

VL - 24

SP - 19

EP - 29

JO - American Journal of Chinese Medicine

JF - American Journal of Chinese Medicine

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ER -

Modulation of microsomal cytochrome P450 by Scutellariae Radix and Gentianae Scabrae Radix in rat liver

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