TY - JOUR
T1 - Modulation of calcium signaling by nanosecond electric pulses and cell death through apoptosis in A549 lung cancerous cells
AU - Awasthi, Kamlesh
AU - Chang, Feng Lin
AU - Wu, Tsai En
AU - Hsu, Hsin-Yun
AU - Ohta, Nobuhiro
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/10/15
Y1 - 2022/10/15
N2 - Calcium ion (Ca2+), which serves as one of the important signaling agents, is imperative to control normal cell function. The altered Ca2+ signaling is known to play a crucial role in the resistance of cancerous cells against apoptosis. Herein, nanosecond pulsed electric field (nsPEF) was applied to non-small lung cancerous cells A549, and Ca2+ mobilization along with mitochondria-mediated apoptotic cell death that accompanies morphological changes was investigated. In the presence of nsPEF, intracellular mobilization of Ca2+ ions through the efflux from endoplasmic reticulum storage was dependent on the pulse-width of the applied field. The field-induced Ca2+ mobilization has shown to correlate with the superoxide anion (O2−) generation, which probably occurred in mitochondria utilizing the nicotinamide adenine dinucleotide (NADH), as supported by the field-induced change in autofluorescence intensity and lifetime of NADH. Mitochondrial dysfunction and O2− generation, which promoted apoptotic cell death, were also induced by nsPEF with strong correlation with intracellular Ca2+ ions.
AB - Calcium ion (Ca2+), which serves as one of the important signaling agents, is imperative to control normal cell function. The altered Ca2+ signaling is known to play a crucial role in the resistance of cancerous cells against apoptosis. Herein, nanosecond pulsed electric field (nsPEF) was applied to non-small lung cancerous cells A549, and Ca2+ mobilization along with mitochondria-mediated apoptotic cell death that accompanies morphological changes was investigated. In the presence of nsPEF, intracellular mobilization of Ca2+ ions through the efflux from endoplasmic reticulum storage was dependent on the pulse-width of the applied field. The field-induced Ca2+ mobilization has shown to correlate with the superoxide anion (O2−) generation, which probably occurred in mitochondria utilizing the nicotinamide adenine dinucleotide (NADH), as supported by the field-induced change in autofluorescence intensity and lifetime of NADH. Mitochondrial dysfunction and O2− generation, which promoted apoptotic cell death, were also induced by nsPEF with strong correlation with intracellular Ca2+ ions.
KW - Apoptotic cell death
KW - Intracellular Ca imaging
KW - Mitochondrial dysfunction
KW - NADH autofluorescence lifetime imaging
KW - Nanosecond pulsed electric field effect
KW - Reactive superoxide anion
UR - http://www.scopus.com/inward/record.url?scp=85134739630&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2022.132348
DO - 10.1016/j.snb.2022.132348
M3 - Article
AN - SCOPUS:85134739630
SN - 0925-4005
VL - 369
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 132348
ER -