Miniaturized video-rate epi-third-harmonic-generation fiber-microscope

Shih Hsuan Chia, Che Hang Yu, Chih Han Lin, Nai Chia Cheng, Tzu Ming Liu, Ming Che Chan, I. Hsiu Chen, Chi Kuang Sun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


With a micro-electro-mechanical system (MEMS) mirror, we successfully developed a miniaturized epi-third-harmonic-generation (epi-THG) fiber-microscope with a video frame rate (31Hz), which was designed for in vivo optical biopsy of human skin. With a large-mode-area (LMA) photonic crystal fiber (PCF) and a regular microscopic objective, the nonlinear distortion of the ultrafast pulses delivery could be much reduced while still achieving a 0.4μm lateral resolution for epi-THG signals. In vivo real time virtual biopsy of the Asian skin with a video rate (31Hz) and a sub-micron resolution was obtained. The result indicates that this miniaturized system was compact enough for the least invasive hand-held clinical use.

Original languageEnglish
Pages (from-to)17382-17391
Number of pages10
JournalOptics Express
Issue number16
StatePublished - 2 Aug 2010


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