Miniaturized parallelized sandwich immunoassays

Hsin-Yun Hsu, Silke Wittemann, Thomas O. Joos

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

17 Scopus citations


This chapter describes the development and use of bead-based miniaturized multiplexed sandwich immunoassays for focused protein profiling. Bead-based protein arrays or suspension microarrays allow simultaneous analysis of a variety of parameters within a single experiment. In suspension, microarrays capture antibodies are coupled onto colorcoded microspheres. The applications of suspension microarrays are described, which allow to analyze proteins present in different types of body fluids, such as serum or plasma, cerebrospinal, pleural and synovial fluids, as well as cell culture supernatants. The chapter is divided into the generation of suspension microarrays, sample preparation, processing of suspension, microarrays, validation of analytical performance, and finally pattern generation using bioinformatics tools.

Original languageEnglish
Title of host publicationClinical Proteomics - Methods and Protocols
Number of pages15
StatePublished - Jan 2008

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Biological fluids
  • Cell culture supernatants
  • Cerebrospinal fluid
  • Immunoassay
  • Microspheres
  • Pleura
  • Protein profiling
  • Serum
  • Suspension microarray
  • Synovial fluid


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